Plant extract product composition, and preparation method and application thereof

A plant extract and extract technology, applied in the direction of drug combination, pharmaceutical formula, cosmetic preparation, etc., can solve the problems of insufficient whitening effect, increased safety risk, too many Chinese herbal components, etc., and achieve excellent free radical scavenging. effect, significant tyrosinase inhibitory effect, high free radical scavenging effect

Active Publication Date: 2016-02-24
JALA GROUP CORPORATION
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AI Technical Summary

Problems solved by technology

[0004] Many of the existing patents use a variety of natural plant Chinese herbal medicines as components to make whitening preparations, but on the one hand, these Chinese herbal medicines have too many components, which increases the safety risk of use; on the other hand, they are not effective compared to existing whitening agents such as arbutin protrude

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of plant extract composition of the present invention

[0026] 1. Preparation of Acacia extract:

[0027] After pulverizing 100g of acacia, mix it with 2L of 70v / v% ethanol solution (volume to mass ratio: 50g:1L), conduct ultrasonic extraction for 1 hour at room temperature, separate the crude extract, and distill off the ethanol solution under reduced pressure to obtain gold Albizia Julibrissin Extract 6g.

[0028] 2. Preparation of Paeoniae officinalis extract:

[0029] Dry and pulverize 100g of peony, mix it with 1L of 70% ethanol solution (volume to mass ratio 100g:1L), and conduct ultrasonic extraction for 1 hour at room temperature to separate the extract, and evaporate the ethanol solution under reduced pressure to obtain the peony extract 28.8 g.

[0030] 3. According to the weight ratio of Acacia extract and Paeonia officinalis extract in the ratio of 1:10, 1:2, 1:1, 2:1 and 10:1, plant extract compositions with different propor...

Embodiment 2

[0031] Example 2: Determination of Tyrosinase Inhibitory Activity

[0032] Mix the different proportions obtained in Example 1 to form a plant extract composition that is completely dissolved in deionized water, and then prepare a 0.5 mg / mL aqueous solution for the determination of tyrosinase inhibitory activity, with arbutin as a control , while increasing the composition outside the weight ratio range of (1:10)-(10:1) Acacia extract and Paeoniae officinalis extract as a control.

[0033] The measurement steps are: mix 70 μL of phosphate buffer solution (pH6.8, 30 mM), 80 μL of 1.66 mM tyrosine solution, and 80 μL of sample solution, incubate in a constant temperature water tank at 37°C for more than 5 minutes, add tyrosinase solution (125 U / ml) 10 μL, after constant temperature at 37°C for 10 minutes, measure the absorbance A at 475nm wavelength with a microplate reader 475 . The sample aqueous solution was replaced with deionized water, and the absorbance was also measur...

Embodiment 3

[0039] Embodiment 3: the mensuration of free radical scavenging rate

[0040] Mix the different proportions obtained in Example 1 to form a plant extract composition that is completely dissolved in deionized water, and then be formulated into an aqueous solution of 0.05 mg / mL, and be mixed with DPPH (1,1-diphenyl-2-trinitro Phenylhydrazine) method was used to determine its free radical scavenging antioxidant activity. The determination steps are: pipette 2mL extract solution into a 10mL stoppered test tube, add 2mL DPPH ethanol solution (2×10 -4 mol / L), fully mixed, after standing at room temperature for 30min, measure the absorbance A at 517nm wavelength with a spectrophotometer 517 ; Simultaneously measure the absorbance A of 2mL extract solution mixed with 2mL ethanol 0, the absorbance C after mixing 2mL water and 2mL DPPH ethanol solution and the absorbance C after mixing 2mL water and 2mL ethanol solution 0 . Three parallel measurements were taken, the average value w...

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Abstract

The invention relates to the technical field of plant extraction, and discloses a plant extract product composition, and a preparation method and an application thereof. The plant extract product composition is composed of a Sweet Acacia extract product and a Paeonia lactiflora extract product. Sweet Acacia and Paeonia lactiflora are selected from many plant sources and are used as compounds, ethanol extract products of the Sweet Acacia and Paeonia lactiflora are mixed, and systemic effects of all active components in the two extract products are used, so a substantial tyrosinase inhibition effect is realized by using few components, and the plant extract product composition has a excellent free radical removal effect, can be applied in preparation of tyrosinase inhibitors, whitening cosmetics and antioxidants, and can be applied in skin whitening.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a plant extract composition and its preparation method and application. Background technique [0002] Skin color is one of the criteria of people's aesthetics. For Asian women, fair complexion is a common pursuit. To this end, various products have emerged, such as: whitening products, UV protection products and film products. After research, skin color is determined comprehensively by various pigments contained in skin cells. Among the many pigments, the amount of melanin contained in melanocytes is the most important determinant. Further studies confirmed that tyrosinase (EC1.14.18.1) is a key enzyme for melanin synthesis. Therefore, by inhibiting the activity of tyrosinase, to reduce the production of melanin, so as to achieve the effect of whitening skin care has become one of the important basis for research and development of skin care products (Journal of Prac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/02A61P17/18
CPCA61K8/97A61K2800/5922A61K2800/782A61Q19/02
Inventor 方兆华李慧
Owner JALA GROUP CORPORATION
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