Method for preparing thermal thermophilus fermentation product by using rice bran enzyme hydrolysate
A technology of thermophilic bacteria and enzymatic hydrolyzate, which is applied in the field of microorganisms, can solve the problems that the products of thermophilic bacteria do not fully play their role, no fermentation products of thermophilic bacteria are seen, and the process is complicated, so as to improve the DPPH Free radical scavenging rate, comprehensive high-value utilization, simple and easy-to-operate fermentation conditions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0040] Embodiment 1 Thermus thermophilus single bacteria fermentation prepares extracellular fermentation product
[0041] The rice bran is gelatinized and hydrolyzed by saccharifying enzyme to obtain an enzymatic hydrolyzate, and after the enzymatic hydrolyzate is suction filtered, the supernatant is taken for steam sterilization for later use. After activation of Thermus thermophilus, 10% of the inoculum was transferred into the rice bran enzyme hydrolyzate fermentation medium (pH 7.8) to carry out fermentation culture, and cultured at 60° C. and 150 rpm for 18 hours.
[0042] After the fermentation, the solid-liquid separation is carried out by centrifugation, and the precipitate and the fermentation liquid are respectively collected, and the fermentation liquid is the extracellular product of the fermentation of Thermus thermophilus. The test results of DPPH free radical scavenging rate showed that the DPPH free radical scavenging rate of the extracellular product fermente...
Embodiment 2
[0044] Example 2 Thermus thermophilus and yeast combined fermentation to prepare extracellular fermentation products
[0045] The rice bran is gelatinized and hydrolyzed by saccharifying enzyme to obtain an enzymatic hydrolyzate, and after the enzymatic hydrolyzate is suction filtered, the supernatant is taken for steam sterilization for later use. After activating the yeast and Thermus thermophilus, the yeast was first inserted into the rice bran enzymatic hydrolyzate (pH 7.8) with 10% inoculum, and the fermentation was carried out. After the pH of the liquid was adjusted to 7.8, the Thermus thermophilus was inoculated with 10% of the inoculum, and the fermentation was continued for 18 hours at 60° C. and 150 rpm.
[0046] After the fermentation, the solid-liquid separation was carried out by centrifugation, and the precipitate and the fermentation broth were collected respectively. The fermentation broth was the extracellular product of Thermus thermophilus fermentation. Th...
Embodiment 3
[0048] Example 3 Thermus thermophilus and yeast combined fermentation to prepare fermentation product
[0049] The rice bran is gelatinized and hydrolyzed by saccharifying enzyme to obtain an enzymatic hydrolyzate. After the enzymatic hydrolyzate is suction filtered, 10 g / L of nitrogen source (yeast extract powder) is added to the rice bran enzymatic hydrolyzate, and the supernatant is steam sterilized for later use. After activating the yeast and Thermus thermophilus respectively, the yeast was first inserted into the rice bran enzymatic hydrolyzate (pH 7.8) with a 10% inoculation amount, and fermented and cultured at 30 ° C and 200 rpm for 12 After 10 hours, the pH of the fermentation broth was adjusted to 7.8; then 10% of the inoculum was inoculated with Thermus thermophilus, and the fermentation was continued for 24 hours at 60° C. and 150 rpm. After the fermentation is completed, solid-liquid separation is performed to obtain a fermentation broth.
[0050] The above ferm...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com