Method for degrading sterol compounds in tobacco extract with biological method

A technology of leachate and sterols, applied in the field of sterols harmful compounds, can solve the problems of loss of fragrance substances and latent substances, few sterols, and unsatisfactory effects, etc., and achieves high originality, simple and fast process. Effect

Active Publication Date: 2016-02-24
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of removing sterol compounds in tobacco by extraction method is not very satisfactory, and it may lead to the loss of some important aroma substances and latent substances in tobacco
The meth

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for degrading sterol compounds in tobacco extract with biological method
  • Method for degrading sterol compounds in tobacco extract with biological method
  • Method for degrading sterol compounds in tobacco extract with biological method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] A biological method for degrading sterols in tobacco leachate, and its steps are as follows:

[0024] (1) Isolation of sterol-lowering microorganisms: Tobacco leaves are from Xiang County, Henan Province. After the tobacco leaves are destemmed, the leaves are cut into 2cm*2cm small leaves, washed twice with sterile deionized water, and the precipitate is collected and used 15ml pH7.0 Dilute the precipitate with phosphate buffer solution, inoculate it into the separation medium according to the 10% inoculum amount, incubate in a shaker at 37°C at 150r / min, and after enrichment for 2 times, take 200μl of the culture solution and apply it on the solid plate. A single colony of microorganisms that can degrade sterols. The raw materials of the separation medium are: stigmasterol 7412.1g; sorbitan monooleate polyoxyethylene ether (tween80) 0.9ml; citric acid 2.1g; K 2 HPO 4 0.4g; NH 4 Cl 1.1g; add water to 0.9L.

[0025] (2) Microbial expansion culture: pick the single colony in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for degrading sterol compounds in tobacco extract with a biological method. According to the method, sterol degrading bacteria are separated from surfaces of tobacco by the aid of a culture medium, expansion culture of microorganisms is performed by the aid of a seed culture medium, cells at the logarithmic phase are collected, the tobacco extract is inoculated with the cells, and fermentation is performed. With the adoption of the method, the microorganisms for degrading sterols are separated from the surfaces of the tobacco, after expansion is performed by the aid of the seed culture medium, the cells at the logarithmic phase are acquired and added to the tobacco extract, the sterols in the tobacco extract can be effectively degraded, and the total degradation rate of the sterols is up to 35.5%. The sterols are precursor compounds of a hazardous substance, namely, benzopyrene, of the tobacco, and the method provides a reference for biological harm reduction for the tobacco, has the very high originality and is expected to be applied to the reconstituted tobacco production process capable of reducing the benzopyrene.

Description

technical field [0001] The invention belongs to the field of tobacco biotechnology, and in particular relates to a method for degrading harmful sterol compounds in tobacco by using a biological method. Background technique [0002] Sterols belong to macromolecular alcohols and are a class of steroidal compounds that widely exist in nature. They mainly exist in fats or oils of animals and plants, or exist in animals as esters of higher fatty acids or in the form of glycosides in plants. organization. Its basic skeleton structure is a cyclopentahydrophenanthrene system, which consists of three six-membered rings and one five-membered ring (as shown in the figure below). There may be hydrocarbon groups or keto groups at C-3 and C-11 positions. Each of the C-13 positions has a methyl group, and the C-17 position has a side chain composed of 8 to 10 carbon atoms. [0003] [0004] Sterols are a class of hormone substances in tobacco plants, which can promote the growth of to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A24B15/20
CPCA24B15/20C12N1/20
Inventor 叶建斌杨雪鹏郝辉闫记刘向真杨宗灿马科毛多斌
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap