Content determination method for scutellarin and scutellarein in sculellaria barbata medicinal material and formula granule of sculellaria barbata medicinal material
A technology of scutellarein and scutellarein, which is applied in the field of content determination of scutellarein and scutellarein in Scutellaria barbata and its formula granules, which can solve the problems of not being searched and ensure accuracy and reproducibility Effect
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Embodiment 1
[0044] (1) Chromatographic conditions and system suitability test Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 12.5:15:72.5 is used as the mobile phase; column temperature: 30°C; detection wavelength is 340±2nm; Glycoside peak calculation should not be less than 3000;
[0045] (2) Preparation of reference substance solution Take appropriate amount of scutellarein and scutellarein reference substance respectively, accurately weigh them, put them in a brown measuring bottle, add 70% methanol to make each 1ml contains 40 μg of scutellarin and 12 μg of scutellarein The solution, as the reference substance solution;
[0046] (3) Preparation of the test solution Take 0.5g of Scutellaria barbata medicinal material fine powder, accurately weighed; or 0.1g of Scutellaria barbata formula granules, accurately weighed; respectively put in conical flasks with stoppers, and accurately add 70% methanol 25ml, densely packed, weighed, the fine powder of medicinal material...
Embodiment 2
[0049] (1) Chromatographic conditions and system suitability test Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 12.5:16.2:71.3 is used as the mobile phase; column temperature: 30°C; detection wavelength is 340±2nm; Glycoside peak calculation should not be less than 3000;
[0050] (2) Preparation of reference substance solution Take appropriate amount of scutellarein and scutellarein reference substance respectively, accurately weigh them, put them in a brown measuring bottle, add 70% methanol to make each 1ml contains 40 μg of scutellarin and 12 μg of scutellarein The solution, as the reference substance solution;
[0051] (3) Preparation of the test solution Take 0.5g of Scutellaria barbata medicinal material fine powder, accurately weighed; or 0.1g of Scutellaria barbata formula granules, accurately weighed; respectively put in conical flasks with stoppers, and accurately add 70% methanol 25ml, densely packed, weighed, the fine powder of medicinal materi...
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