Use of niclosamide phosphate for preparing medicine for inhibiting renal tissue fibrosis
A technology of nisalamide phosphate and tissue fibrosis, which can be applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve problems such as lack of renal fibrosis
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[0029] Example 1: Inhibition experiment of niclosamide phosphate (p-NICLO) on renal fibrosis in UUO mice
[0030] 1. Experimental animals: BALB / c mice, male, weighing 23-25g, SPF grade. The animals were first weighed and numbered, and 15 healthy mice weighing 23-25 g were selected and randomly divided into 3 groups with 5 mice in each group. Including sham operation group, positive control group and medication group.
[0031] 2. Treatment of each group
[0032] (1) Sham operation group: at room temperature, after anesthetized with 0.05ml / 10g of 1.5% pentobarbital sodium, choose the incision 1-2cm below the left dorsal costal margin; after local disinfection, cut the skin, subcutaneous, muscle Layers and peritoneum were found, and the left ureter was sutured layer by layer. After local disinfection, check the marks and place them in corresponding mouse cages.
[0033] (2) Positive control group: Same as above for anesthesia and disinfection. The skin, subcutaneous layer,...
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