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Megalobrama amblycephala hypoxia character correlation SNP molecular markers and application thereof

A technology of molecular markers and bream, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problems that have not been seen, the decrease of CASP8 cell death rate, and the decrease of mRNA expression

Inactive Publication Date: 2016-03-02
HUAZHONG AGRI UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Sun et al. (2007) found that the -6526N deletion in the promoter region of the CASP8 gene was significantly associated with a reduction in the risk of lung cancer through association analysis. At the same time, they conducted functional verification of SNPs and found that the mRNA expression level corresponding to the CASP8 gene decreased due to the -6526N deletion. The decreased activity of CASP8 in T lymphocytes and the reduction of cell death induced by cancer cell antigens
However, there is no report on the SNP molecular markers of hypoxia-related genes in bream

Method used

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  • Megalobrama amblycephala hypoxia character correlation SNP molecular markers and application thereof
  • Megalobrama amblycephala hypoxia character correlation SNP molecular markers and application thereof
  • Megalobrama amblycephala hypoxia character correlation SNP molecular markers and application thereof

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Embodiment 1

[0051] 1.1 Test animals

[0052] The bream used comes from Hubei Tuanfeng Aquatic Fine Breed Company. 1,000 1-year-old bream of about 100 g were randomly selected from groups living in the same environment, and placed in a 2m×2m×2m water-filled cement pool for a while. After raising for 3 days, the hypoxic treatment experiment was carried out. The process of hypoxic treatment is as follows: turn off the water filling device, the dissolved oxygen level in the pool drops from 6.50 mg / L to 1.00 mg / L within two hours, take 100 breams with the first floating head and the last floating head, respectively. As hypoxia-sensitive and hypoxia-tolerant populations of bream.

[0053] 1.2 Test method

[0054] 1.2.1 Extraction of genomic DNA and RNA from bream

[0055] The fin ray tissues of two populations of bream were taken, and genomic DNA was extracted according to the traditional phenol-form extraction method, and the obtained DNA samples were stored at -20°C. At the same time, 5 fi...

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Abstract

The invention belongs to the technical field of ichthyic molecular marker preparation, and particularly relates to megalobrama amblycephala hypoxia character correlation SNP molecular markers and the application thereof. The screened-out megalobrama amblycephala fih-1 gene SNPs molecular markers are located at different positions of the fih-1 gene. The sequence of the SNP molecular markers in a promoter is shown in the SEQ ID NO:1, and an allelic variant exists at the position of 109bp of the sequence. The sequence of the SNP molecular markers in 5'UTR is shown in the SEQ ID NO:2, and an allelic variant exists at the position of 337bp of the sequence. The sequence of the SNP molecular markers in 3'UTR is shown in the SEQ ID NO:3, and an allelic variant exists at the position of 87bp of the sequence. The screened-out megalobrama amblycephala hypoxia tolerance related gene polymorphic SNPs molecular markers can be used for marker assisted selection of megalobrama amblycephala.

Description

technical field [0001] The invention belongs to the field of preparation of fish molecular markers, and in particular relates to a SNP molecular marker and application of the hypoxia-related gene fih-1 of bream. The molecular marker can be applied to auxiliary selection of breeding genotypes of bream. Background technique [0002] Megalobrama amblycephala, commonly known as Wuchang fish and bream, belongs to Cyprinidae, Cyprinidae, genus Bream, and is one of the important herbivorous economic fishes in my country. However, compared with typical carps such as grass carp, carp, and crucian carp, bream is not resistant to hypoxia, and is prone to hypoxic floating heads, which restricts the improvement of its aquaculture production. Different individuals in the same group have different tolerance to hypoxic environment. Therefore, the cultivation of new low-oxygen resistant strains of bream is of great significance to the development of bream aquaculture. [0003] Hypoxia-indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王焕岭张豹陈伯湘陈楠王卫民刘红
Owner HUAZHONG AGRI UNIV
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