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Preparation method for grateloupia filicina polypeptide solution

The technology of centipede algae and polypeptide liquid is applied in the preparation field of using centipede algae to prepare centipede algae polypeptide liquid, can solve the problems such as the preparation technology of centipede algae polypeptide has not been found, achieve high extraction rate and purity, improve extraction rate and simple operation. Effect

Inactive Publication Date: 2016-03-09
RONGCHENG GUANGRUN SEAFOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the preparation process of centipede algae polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of centipede algae polypeptide liquid, it goes through the following process steps:

[0020] (1) Raw material pretreatment: select fresh centipede algae as raw material, dry it at 50°C and then pulverize it to obtain dry centipede algae powder; add centipede algae dry powder to deionized water with a solid-to-liquid ratio of 1:15, and store at 4°C Soak overnight to make it fully swell, then use a grinder to control the speed of 2000r / min, grind for 80min, and make a centipede algae homogenate;

[0021] (2) One-time enzymatic hydrolysis: quantitatively weigh the centipede algae homogenate obtained in step (1) and add trypsin with 1.5% of centipede algae dry powder quality, the enzyme activity> 15000ul / g, mix well; use 1mol / l sodium carbonate solution Adjust the pH value of the solution to 8, control the temperature at 37°C, and perform enzymatic hydrolysis for 3 hours to obtain an enzymatic hydrolysis solution; use a centrifuge to control the speed o...

Embodiment 2

[0028] A preparation method of centipede algae polypeptide liquid, it goes through the following process steps:

[0029] (1) Raw material pretreatment: select fresh centipede algae as raw material, dry it at 60°C and then pulverize it to obtain dry centipede algae powder; add centipede algae dry powder to deionized water, the ratio of solid to liquid is 1:20, and store at 4°C Soak overnight to make it fully swell, then use a grinder to control the speed of 3000r / min, grind for 60min, and make a centipede algae homogenate;

[0030] (2) primary enzymolysis: the centipede algae homogenate that obtains in step (1) is added the protease of centipede algae dry powder quality 2%, wherein, protease is by the papain of 40%, the neutral protease of 30%, the protease of 20% Alkaline protease is mixed, and the enzyme activity is >20000ul / g; use 1mol / l sodium carbonate solution to adjust the pH value of the solution to 9, control the temperature at 35°C, and enzymatically hydrolyze for 3 h...

Embodiment 3

[0037] A preparation method of centipede algae polypeptide liquid, it goes through the following process steps:

[0038] (1) Raw material pretreatment: select fresh centipede algae as raw material, dry it at 55°C and then pulverize it to obtain dry centipede algae powder; add centipede algae dry powder to deionized water with a solid-to-liquid ratio of 1:10, and store at 4°C Soak overnight to make it fully swell, then use a grinder to control the speed of 6000r / min, grind for 30min, and make a centipede algae homogenate;

[0039] (2) One-time enzymatic hydrolysis: the centipede algae homogenate obtained in step (1) is added protease of 1% of centipede algae dry powder quality, wherein, protease is made of 30% papain, 20% bromelain, 50% stomach Protease mixed, enzyme activity > 20000ul / g; use 1mol / l sodium carbonate solution to adjust the pH value of the solution to 3, control the temperature at 60°C, and enzymatically hydrolyze for 2.5 hours to obtain a primary enzymatic hydro...

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PUM

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Abstract

The invention discloses a preparation method for a grateloupia filicina polypeptide solution. According to the method, fresh grateloupia filicina is used as the raw material and subjected to drying, smashing, soaking in water and grinding to obtain uniform grateloupia filicina slurry; a certain amount of protease is added to the uniform grateloupia filicina slurry, and enzymolysis conditions are controlled for two times of enzymolysis to obtain an enzymolysis solution; the enzymolysis solution is subjected to separation and purification through a gel chromatographic column after enzyme deactivation and centrifugal treatment, and then a purified grateloupia filicina polypeptide stock solution is obtained. The preparation method is reasonable in process and easy to operate, the extraction rate and purity of grateloupia filicina polypeptide are high, and the preparation method is suitable for large-scale production.

Description

technical field [0001] The invention relates to the preparation of peptides, in particular to a preparation method for using centipede algae to prepare centipede algae polypeptide liquid. Background technique [0002] We know that centipede algae, also known as sea red cabbage, Dongjialan, and sundew, is a plant of the genus Centipede algae in the family Centipedeceae of the Rhodophyta family. It has the effects of clearing away heat, detoxifying and repelling insects. The body contains L-Cantyl-L-Canthenic Acid, Centipede Alginic Acid, Taurine, Agar, Polysaccharide, Protein and Sulfate Steroid, Phosphate, and other trace elements. Studies on centipede algae have shown that its composition is complex and has a variety of biological activities. It has the functions of preventing and treating various cardiovascular diseases, anti-tumor, anti-coagulation, anti-thrombosis, anti-virus, anti-oxidation and enhancing immunity. In recent years, the research on centipede algae mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/16
CPCC12P21/06C07K1/16
Inventor 杨劲峰吴文惠付强
Owner RONGCHENG GUANGRUN SEAFOOD CO LTD
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