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Application of pemetrexed disodium or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection

A technology for pemetrexed disodium and herpes virus infection, which is applied in the field of biomedicine and achieves good anti-herpes virus infection effect

Inactive Publication Date: 2018-01-09
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, drugs to treat or prevent herpes virus infection still need to be further developed

Method used

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  • Application of pemetrexed disodium or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection
  • Application of pemetrexed disodium or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection
  • Application of pemetrexed disodium or pharmaceutically acceptable salt thereof in preparation of medicine for treating or preventing herpes virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Detection of pemetrexed disodium inhibiting KSHV lytic phase replication

[0038] 1. Experimental materials

[0039] 1.1 Cells and viruses

[0040] iSLK.219 cells were donated by Professor Dr. Don Ganem. The cells are SLK cells containing recombinant virus rKSHV.219 and doxycycline (Doxycycline) regulated RTA expression system. In this cell, the RTA expression sequence was constructed into the pRetro-X Tet-ON inducible expression system (Clontech, Mountainview, CA), and the expression of this system was regulated by doxycycline. In the presence of doxycycline, RTA expression can occur; 293T cells were preserved in our laboratory and purchased from American Type Culture Collection (ATCC).

[0041] Pemetrexed disodium was purchased from Biochempartner.

[0042] 1.2 Reagents

[0043] DMEM medium, 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal Green Supermix) was purchased f...

Embodiment 2

[0104] Detection of pemetrexed disodium inhibiting DNA replication in EBV lytic phase

[0105] 1. Experimental materials

[0106] 1.1 Cells and viruses

[0107] The B95-8 cell was donated by Professor Sun Xiaoping of the School of Basic Medicine of Wuhan University. The cell contains the complete sequence of the EBV virus genome. Combined induction with 20ng / mL TPA can make the virus enter the lytic phase and replicate.

[0108] 1.2 Reagents

[0109] 1640 medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; SYBR mixture (iTaq TM Universal Green Supermix) was purchased from Bio-Rad Company.

[0110] 1.3 Experimental Instruments

[0111] The multi-marker microplate reader was purchased from PerkinElmer; the fluorescent quantitative PCR instrument (Bio-RadCFX96Touch TM Real-Time PCR detection system) was purchased from Bio-Rad Company; 1.0R refrigerated centrifuge and cell culture box were purchased from Thermo Company....

Embodiment 3

[0134] Embodiment 3: the detection of pemetrexed disodium anti-HSV-1 and HSV-2 activity

[0135] 1. Experimental materials

[0136] 1.1 Cells and viruses

[0137] Vero cells were preserved in our laboratory and purchased from the American Type Culture Collection (ATCC). HSV-1 is an F strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences. HSV-2 is a G strain, which is preserved by the Wuhan Institute of Virology, Chinese Academy of Sciences.

[0138] 1.2 Reagents

[0139] DMEM medium and FBS were purchased from GIBCO; Alamarblue activity detection kit was purchased from Promega; crystal violet staining solution.

[0140] 1.3 Experimental Instruments

[0141] The multi-label microplate reader and the high-content cell analyzer were purchased from PerkinElmer; the cell incubator was a product of Thermo.

[0142] 2. Experimental methods and results

[0143] 2.1 Cytotoxicity detection of pemetrexed disodium on Vero cells.

[0144] (1) Di...

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Abstract

The invention discloses application of pemetrexed disodium or pharmaceutically-acceptable salt thereof in preparing medicine for treating or preventing herpes virus infection. According to the application, pemetrexed disodium of a completely non-cytotoxic concentration is selected and used for conducting a herpes virus resisting experiment, the result shows that the compound has significant herpes virus resisting activity in dose dependency, and pemetrexed disodium performs the herpes virus resisting effect by inhibiting virus reproduction. Detection of herpes viruses of different subtypes shows that pemetrexed disodium has broad-spectrum herpes virus resisting activity, is high in selection index, can be developed into medicine for treating or preventing herpes virus infection and has broad application prospects.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of pemetrexed disodium or a pharmaceutically acceptable salt thereof in the preparation of medicines for treating or preventing herpes virus infection. Background technique [0002] Herpesviridae is a family of viruses with envelopes and double-stranded DNA genomes. Members of this family can widely infect animals and humans and induce corresponding diseases. There are currently eight herpes viruses that can infect humans, mainly including: Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Varicella zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpes virus type VI (HHV-6), human herpes virus type VII (HHV-7) and Kaposi's sarcoma-associated herpesvirus (KSHV). According to the differences in genome sequence, structure and physical and chemical properties, Herpesviridae can be divided into three subfamilies: α-Herpesvirinae, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/519A61P31/22
CPCA61K31/519
Inventor 陈绪林陈军刚
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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