Microalgae breeding method with high CO2 tolerance and fixed rate

A technology of tolerance and fixation rate, applied in the field of microalgae breeding with high CO2 tolerance and fixation rate, can solve the problems of low carbon fixation efficiency, poor high temperature resistance of microalgae, and high technical complexity

Pending Publication Date: 2016-03-23
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

How to take cost-effective methods to control CO under the existing fuel and energy structure conditions 2 Emissions have been a key research topic for researchers for many years; according to the research experience of many scientists and the analysis of the applicant's research results in recent years, the microbial method is an effective emission reduction m

Method used

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  • Microalgae breeding method with high CO2 tolerance and fixed rate
  • Microalgae breeding method with high CO2 tolerance and fixed rate
  • Microalgae breeding method with high CO2 tolerance and fixed rate

Examples

Experimental program
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Effect test

Embodiment 1

[0062] The high CO involved in this example 2 The breeding method of tolerance and fixation rate microalgae specifically comprises the following steps:

[0063] (1) Sample collection and pre-culture:

[0064] The test samples are: Fujian paddy field soil samples (moisture content ≧ 90%), water samples from a pond in Wenquan Town, Qingdao, water samples from a natural river in Wenquan Town, Qingdao, water samples from sewage ponds in Wenquan Town, Qingdao, water samples from pools near Qingdao Power Plant and Water samples from Qingdao Haibohe Power Plant section;

[0065] Before culturing, the above water samples were observed under an optical biological microscope (LEICA, Shanghai), and the types of microalgae contained were recorded respectively; Inoculate the BBM medium of microalgae with a wet weight of about 1g into the medium, and cultivate it in a light incubator (SPX-250B, Shanghai Yuejin Medical Instrument Factory). The control culture condition is: 25°C ±2°C, ligh...

Embodiment 2

[0123] In this example with high CO 2 The microalgae breeding method of tolerance and fixation rate is specifically selected and cultivated in a microalgae breeding device, and the main structure of the microalgae breeding device includes CO 2 Steel cylinder 1, air pump 2, CO 2 Analyzer 3, water bath heater 4, fiber membrane module 5, reactor 6, light source 7, three-way valve 8, gas flow meter 9, gas distributor 10, No. I pipeline 11, No. II pipeline 12 and No. III pipeline 13 , CO 2 The mouth of steel cylinder 1 is provided with No. 1 pipeline 11 for transporting CO 2 Gas, the air pump 2 is connected with the No. II pipeline 12 for transporting air, the No. II pipeline 12 communicates with the No. I pipeline 11 through the three-way valve 8, and the No. I pipeline 11 is connected with a gas distributor 10 for preparing CO 2 Cylinder 1 and air pump gas to form CO-enriched 2 The gas; the No. I pipeline 11 connected to the lower end of the gas distributor 10 passes through ...

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Abstract

The invention belongs to the technical field of microalgae breeding, and particularly relates to a microalgae breeding method with high CO2 tolerance and fixed rate. The method specifically comprises the steps of sample collection and preculture, simulated flue gas habituated culture, flat separation, relatively pure population collection, pure population acquisition, purebred saving, cultural character testing, high-intensity habituated culture, gene mutation breeding, passage stability testing, gene cloning and sequencing, algal strain carbon content testing, target strain cultural character testing, and optimum target strain determination. Cultivated strain has the CO2 tolerance under the maximal condition. The whole method is scientific in principle, the adopted steps are clear, operation is easy, and conditions are controllable.

Description

Technical field: [0001] The invention belongs to the technical field of microalgae cultivation, and relates to a method for selecting and cultivating microalgae, in particular to a method with high CO 2 Microalgae breeding method for tolerance and fixation rate, capable of cultivating high CO 2 Tolerance and fixation rates of microalgae. Background technique: [0002] Since the industrial revolution, the carbon dioxide and other heat-absorbing greenhouse gases emitted by humans into the atmosphere have increased year by year, and the greenhouse effect of the atmosphere has also increased. A series of problems caused by it have attracted the attention of countries all over the world. How to take cost-effective methods to control CO under the existing fuel and energy structure conditions 2 Emissions have been a key research topic for researchers for many years; according to the research experience of many scientists and the analysis of the applicant's research results in rec...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/12C12N1/02C12N13/00C12N15/01C12M1/00C12M1/34C12M1/04
CPCC12N1/36C12M21/02C12M29/04C12M29/06C12M41/22C12M41/44C12N1/02C12N1/12C12N13/00C12N15/01
Inventor 岳丽宏陈为公
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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