Application of endophytic fungus sdte-p in the control of Panax notoginseng black spot

A technology of Panax notoginseng black spot and endophytic fungus, applied in the biological field, can solve problems such as pollution, threats to health, production reduction of Panax notoginseng, etc., and achieve broad application prospects and strong inhibitory effect

Active Publication Date: 2019-06-21
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even failure of Sanqi, and brought huge economic losses to the growers.
In order to control this fungal disease, a large amount of chemical pesticides are used, resulting in a large amount of pesticide residues in Panax notoginseng products, seriously polluting the environment, and greatly threatening people's health

Method used

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  • Application of endophytic fungus sdte-p in the control of Panax notoginseng black spot
  • Application of endophytic fungus sdte-p in the control of Panax notoginseng black spot
  • Application of endophytic fungus sdte-p in the control of Panax notoginseng black spot

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation, screening and identification of strains

[0024] 1. Isolation of strains

[0025] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0026] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0027] Medium: 1000ml potato dextrose agar medium (PDA medium), PDA medium contains 300g potato, 20g glucose, 20g agar and 0.1g chloramphenicol, the pH value of the medium is 6.0±0.2.

[0028] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, soak the root of Sophora japonica in 75% ethanol for 1 min, rinse with sterile water twice, soak in sodium hypochlorite (available chlorine 1%) f...

Embodiment 2

[0074] The Inhibitory Effect of the Metabolites of the Strain on Pseudomonas notoginseng

[0075] 1. Fermentation of strains and extraction of metabolites

[0076] (1) Inoculate the endophytic fungus SDTE-P of Sophora vietnamese on a flat plate of potato dextrose agar medium (PDA plate), and culture it at 28°C for 10 days. The obtained culture material is cut into pieces and transferred to a sterile Solid medium (containing 400g potato, 20g dextrose and 20g sucrose) in a 2-liter Erlenmeyer flask, fermented at 28°C for 40 days;

[0077] (2) After the fermentation is completed, soak the fermented product with twice as much methanol as the fermented product and sonicate it for 40 minutes, then filter it with gauze, and take the filtrate;

[0078] (3) Repeat step (2) twice, combine the two filtrates and concentrate under reduced pressure to form an extract, and obtain the crude methanol extract of the endophytic fungus SDTE-P fermentation product, which is the metabolite of the e...

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Abstract

The invention discloses a Vietnamese Sophora japonica endophyte SDTE‑P, which is classified as Penicillium citrinum SDTE‑P, and the ITS sequence of the strain is as described in SEQ ID NO.1 , Preservation Unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, Preservation Address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Preservation Date: May 13, 2015, Preservation Number: CGMCC No. 10808. In the present invention, for the first time, an endophytic fungus (Penicillium citrinum) SDTE‑P is isolated and screened from the root of the medicinal plant Phytophthora citrinum. The field of prevention and treatment brings broad application prospects.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to the application of SDTE-P, an endophytic fungus of Sophora japonica, in the prevention and treatment of Panax notoginseng black spot. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective methods of disease control, and the use of biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Panax notoginseng ( Panax notoginseng F. H. chen ), also known as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P3/00C12R1/80
CPCA01N63/30C12P1/02C12N1/145C12R2001/80
Inventor 李良波姚裕群黄荣韶鲁璇
Owner GUANGXI UNIV
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