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Simultaneous detection method of many samples

A multi-sample, sample technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of not being able to detect standard samples at the same time, inconvenient to carry, etc. Accuracy and detection efficiency, eliminating complicated procedures and improving accuracy

Inactive Publication Date: 2016-04-06
BEIJING PURKINJE GENERAL INSTR
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of photoelectric colorimetry is high accuracy, but the disadvantage is that it cannot detect standard samples and unknown samples at the same time, and it is inconvenient to carry

Method used

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  • Simultaneous detection method of many samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Quantitative analysis is carried out with nitrite test strips, using the first method, the specific process is as follows:

[0039] Step 1: The user places a nitrite test paper standard card and two new unknown samples in the same field of view, and takes pictures, see references figure 1 .1.

[0040] in reference figure 1 .1 is the original picture taken, different cameras have different resolutions, and the pictures taken are of different sizes. figure 1 .1 The resolution is 1936*2592, and the size is 1.17M. For the convenience of calculation, the figure 1 .1 Compressed into a size of no more than 40K (see references figure 1 .2);

[0041] The second step: in the reference material figure 1 .2 Carry out threshold segmentation edge recognition, extract the standard sample area and unknown sample area in the picture, as in the reference figure 1 .3 shown.

[0042] After extracting the weighted gray value of each pixel in the pixel area corresponding to the standa...

Embodiment 2

[0048] The semi-quantitative analysis is carried out by the determination of pesticide residues, and the first method is adopted. The specific process is as follows:

[0049] Step 1: Place three pesticide residue standard cards and a new unknown sample in the same field of view, see references for photos figure 2 .1.

[0050] in reference figure 2 .1 is the original picture of the pesticide residues captured in the reference materials figure 2 .1 The resolution is 2322*4128, for the convenience of calculation, the figure 2 .1 compressed into a resolution of 360*270 (see the reference figure 2 .2).

[0051] The second step: in the reference material figure 2 .2 Perform threshold segmentation to extract the standard sample area and unknown sample area in the picture, as in the reference figure 2 .3 shown.

[0052] The fourth step is to calculate the H, I, S values ​​of the standard sample area. According to the value of the largest difference between H, I, and S i...

Embodiment 3

[0057] Determination of salbutamol with colloidal gold reagent card adopts the first method, and the specific process is as follows:

[0058] Step 1: Take pictures of the albuterol standard card and sample card, as in the reference materials image 3 .1 shown.

[0059] Since the color block of colloidal gold is small, it is easy to cause pixel distortion after compression, so it is necessary to directly intercept the color block information according to the position of the CT line.

[0060] The second step: in the reference material image 3 .1 Carry out threshold segmentation, extract the standard sample area and unknown sample area in the picture according to the position of the CT line, as in the reference material image 3 .2 shown.

[0061] Step 3: Obtain the optimal standard curve.

[0062] The weighted gray value of the standard sample obtained in the above step, H value, I value, the ratio of the respective T line and C line in the S value, according to the semi-lo...

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Abstract

The invention discloses a simultaneous detection method of many samples. The method comprises the following steps: (1) placing many samples into a same field of view and shooting to obtain a color image; (2) carrying out threshold segmentation on the color image and separating samples and the background: 2.1, directly counting number of bacterial colonies if the samples are bacterial colonies; 2.2, carrying out threshold segmentation if the samples include standard samples and unknown samples, separating the standard sample and the unknown samples, and entering Step (3); (3) respectively establishing a corresponding standard curve according to grayscale-weighted threshold, H value, I value or S value of the standard samples; and (4) determining detection results of the unknown samples according to positions of the unknown samples' grayscale-weighted threshold, H value, I value or S value on the standard curve. By the method of the invention, many samples can be detected simultaneously. Therefore, detection flow is shortened, and detection efficiency is raised. The method is more convenient for users to use.

Description

technical field [0001] The invention relates to the technical field of chemical detection by colorimetry, in particular to a method for simultaneous detection of multiple samples. Background technique [0002] Currently, there are two main types of colorimetric detection techniques: visual colorimetry and photoelectric colorimetry. [0003] 1. Visual colorimetry is to qualitatively judge the characteristics of the unknown sample reagent by comparing the difference between the unknown sample and the standard color scale by human eyes. The advantage of this method is that it is easy to operate, the result can be known through observation and comparison after the sample configuration is completed, and multiple standard samples and unknown samples can be observed and detected at the same time. The disadvantage is that the accuracy is low and the subjective error is large, which is suitable for the detection of samples with obvious standard color scale distinctions. [0004] 2....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 陈望朱翠翠
Owner BEIJING PURKINJE GENERAL INSTR