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Lipopeptide and nano-titania compound preparation and preparation method and application thereof

A technology of nano-titanium dioxide and composite preparations, applied in biochemical equipment and methods, botanical equipment and methods, applications, etc., can solve problems such as low yield, limited industrial production of lipopeptides, and limited development.

Active Publication Date: 2016-04-20
NANJING PUSHUAI BIOLOGICAL NANOMETER TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a kind of biosurfactant, lipopeptide surfactant can change the permeability of cell membrane, and has broad-spectrum antifungal and antibacterial activity. Bacteria and R. solanacearum show good antagonistic activity, which can be used to control bacterial wilt, but its chemical instability, physical and enzymatic instability limit the development and application of lipopeptide surfactant-related products
At the same time, higher production costs and lower yields also limit the industrial production of lipopeptides

Method used

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  • Lipopeptide and nano-titania compound preparation and preparation method and application thereof
  • Lipopeptide and nano-titania compound preparation and preparation method and application thereof
  • Lipopeptide and nano-titania compound preparation and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0065] Preparation method of lipopeptide-nano-titanium dioxide composite preparation: (1) Preparation of lipopeptide powder: The cryopreserved Bacillus amyloliquefaciens strain XZ-173 (General Microbiology Center of China Microorganism Culture Collection Management Committee, CGMCC No. 4160) was crossed. On the LB plate, place it in an incubator for activation, pick a single colony and inoculate it in the LB liquid medium, and cultivate it at 30 ° C and 170 rpm for 24 h to obtain a fermented seed liquid; weigh 4.5 kg of soybean meal, 3 kg of straw, 0.15 kg starch and 0.15kg yeast extract were put into a 50L fermenter, 1L inorganic salt solution was added, the pH of the substrate was adjusted to 7, the moisture content was guaranteed to be 40%, and the speed was set to 50rpm and stirred for 60min to fully mix the substrate and sterilize; Among them, the inorganic salt solution includes (g / L): KH 2 PO 4 1.0, MgSO 4 ·7H 2 O0.5, KCl0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 ...

Embodiment 2

[0070] The preparation method of lipopeptide-nano-titanium dioxide composite biocontrol preparation: (1) preparation of lipopeptide powder: the frozen Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain XZ-173 is streaked on the LB plate, placed in the incubator for activation, Pick a single colony and inoculate it in LB liquid medium, cultivate it at 30°C and 170rpm for 24 hours to obtain a fermented seed liquid; weigh 6kg of soybean flour, 4.5kg of straw, 0.2kg of starch and 0.2kg of yeast extract, and put it into 50L In the fermenter, add 2L inorganic salt solution, adjust the pH of the matrix to 8, and the water content to 60%, set the rotation speed to 70rpm and stir for 80min to fully mix the matrix, and sterilize; wherein, the inorganic salt solution includes (g / L): KH 2 PO 4 1.0, MgSO 4 ·7H 2 O0.5, KCl0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3 After the temperature in the fermenter is cooled and kept stable...

Embodiment 3

[0075] The preparation method of lipopeptide-nano-titanium dioxide composite biocontrol preparation: (1) preparation of lipopeptide powder: the frozen Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain XZ-173 is streaked on the LB plate, placed in the incubator for activation, Pick a single colony and inoculate it in LB liquid medium, cultivate it at 30°C and 170rpm for 24 hours, and use it as a fermented seed solution; weigh 5.67kg of soybean flour, 3.67kg of straw, 0.17kg of starch and 0.18kg of yeast extract, and put In a 50L fermenter, add 1L of inorganic salt solution, adjust the pH of the matrix to 7.5, and the water content to 55%, set the rotation speed to 70rpm and stir for 60 minutes to fully mix the matrix and sterilize; wherein, the inorganic salt solution includes (g / L): K H 2 PO 4 1.0, MgSO 4 ·7H 2 O0.5, KCl0.5, L-phenylalanine 2×10 -3 , MnSO 4 5×10 -3 , CuSO 4 0.16×10 -3 , FeSO 4 0.15×10 -3 ; After the temperature in the fermenter is cooled...

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Abstract

The invention discloses a lipopeptide and nano-titania compound preparation. The lipopeptide and nano-titania compound preparation is characterized by being prepared from, by mass, 0.075%-0.15% of lipopeptide, 0.2%-0.3% of nano-titania, 0.0069%-0.0092% of Zn<2+>, 0.005%-0.007% of Fe<2+>, 0.0052%-0.0091% of Cu<2>, 0.005%-0.0083% of Mn<2+> and the balance water. The invention further discloses a preparation method of the lipopeptide and nano-titania compound preparation and application of the lipopeptide and nano-titania compound preparation in preventing and treating tomato bacterial wilt and promoting tomato plant growth. According to the prepared lipopeptide and nano-titania compound preparation, after nano-titania and lipopeptide are combined, the stability of lipopeptide can be improved, the action time of lipopeptide can be prolonged, a good prevention and treatment effect on tomato bacterial wilt can be achieved, and tomato plant growth can be promoted.

Description

technical field [0001] The invention belongs to the field of preparation of microbial pesticides, and relates to a lipopeptide-nano-titanium dioxide composite preparation and a preparation method and application thereof. Background technique [0002] Tomato bacterial wilt is a worldwide widespread bacterial soil-borne disease caused by Ralstonia solanacearum. In the early stage of the disease, the petioles of the lower leaves of the plant drooped, and then the leaves gradually withered, and at the same time adventitious roots appeared on the stems, and the plants wilted rapidly and gradually died. In mild cases, the yield can be reduced by 20% to 40%, and in severe cases, it can reach more than 60% or even no harvest. There is great genetic variation in R. solanacearum, and there is no effective chemical control method at present. Common control measures such as resistance breeding, field clearance, crop rotation, and integrated management have little effect due to the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/16A01N63/02A01N59/20A01P1/00A01P21/00C12P21/00C12R1/07
CPCA01N59/16A01N59/20A01N63/10C12P21/00A01N2300/00
Inventor 冉炜吴彦良和文龙
Owner NANJING PUSHUAI BIOLOGICAL NANOMETER TECH DEV CO LTD
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