Method for rapidly judging malt fermentation performance in beer brewing process
A malting and rapid technology, applied in the direction of instruments, surface/boundary effects, measuring devices, etc., can solve the problems of measurement influence, time-consuming, etc., achieve low cost, simple operation, and eliminate the effects of poor data repeatability defects
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Embodiment 1
[0022] Step 1: Grind malt with a grinder (DLFU disc grinder, the distance between discs is 0.2mm) to obtain 70-80 mesh malt powder; weigh 950mg-1050mg of malt powder into a 2mL centrifuge tube, add 1mL deionized water, 65 Incubate and shake at ℃ for 30min, then raise the temperature to 100℃ for 5min, centrifuge the supernatant at 12000rpm for 5min-10min;
[0023] Step 2: Osmolality detection:
[0024] Take 100uL of the clarified reaction solution and place it in a freezing point osmometer to detect the OC value. The sample is tested three times and the average value is taken;
[0025] Step 3: Determination of Malt Limit Fermentation RDF
[0026] R D F = 0.0176 * O C M 1 * ( 1 - W % ) ...
Embodiment 2
[0031] Step 1: Grind malt with a grinder (DLFU disc grinder, the distance between discs is 0.2mm) to obtain 70-80 mesh malt powder; weigh 950mg-1050mg of malt powder into a 2mL centrifuge tube, add 1mL deionized water, 65 Incubate and shake at ℃ for 30min, then raise the temperature to 100℃ for 5min, centrifuge the supernatant at 12000rpm for 5min-10min;
[0032] Step 2: Osmolality detection:
[0033] Take 100ul of the clarified reaction solution and place it in a freezing point osmometer to detect the OC value. The sample is tested three times and the average value is taken;
[0034] Step 3: Determination of Malt Limit Fermentation RDF
[0035] R D F = 0.0176 * O C M 1 * ( 1 - W % ) ...
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