Method for quickly judging malt fermentation performance in beer brewing process
A malting and rapid technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of measurement influence and time-consuming, and achieve the effect of low cost, simple operation and guaranteed consistency
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Embodiment 1
[0022] Step 1: Grind malt with a grinder (DLFU disc grinder, the distance between discs is 0.2mm) to obtain 70-80 mesh malt powder; weigh 950mg-1050mg of malt powder into a 2mL centrifuge tube, add 1mL deionized water, 65 Incubate and shake at ℃ for 30min, then raise the temperature to 100℃ for 5min, centrifuge the supernatant at 12000rpm for 5min-10min;
[0023] Step 2: Osmolality detection:
[0024] Take 100uL of the clarified reaction solution and place it in a freezing point osmometer to detect the OC value. The sample is tested three times and the average value is taken;
[0025] Step 3: Determination of Malt Limit Fermentation RDF
[0026]
[0027] Among them, RDF is the limit fermentation degree of malt, %, OC is the osmotic pressure value, mosm; M 1 is the weight of the raw material powder weighed, g; W is the moisture contained in the raw material, %
[0028] In order to verify whether the osmotic pressure can be used to evaluate the fermentation performance of ...
Embodiment 2
[0031] Step 1: Grind malt with a grinder (DLFU disc grinder, the distance between discs is 0.2mm) to obtain 70-80 mesh malt powder; weigh 950mg-1050mg of malt powder into a 2mL centrifuge tube, add 1mL deionized water, 65 Incubate and shake at ℃ for 30min, then raise the temperature to 100℃ for 5min, centrifuge the supernatant at 12000rpm for 5min-10min;
[0032] Step 2: Osmolality detection:
[0033] Take 100ul of the clarified reaction solution and place it in a freezing point osmometer to detect the OC value. The sample is tested three times and the average value is taken;
[0034] Step 3: Determination of Malt Limit Fermentation RDF
[0035]
[0036] Among them, RDF is the limit fermentation degree of malt, %; OC is the osmotic pressure value, mosm; M 1 is the weight of the raw material powder weighed, g; W is the moisture contained in the raw material, %
[0037] In order to verify whether the osmotic pressure can be used to evaluate the fermentation performance of ...
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