Rice cyclin OsCYCP4 ; 4 application and method for improving rice tolerance to low phosphate stress

A cell cycle and low phosphorus tolerance technology, applied in the field of plant genetic engineering, can solve problems such as no CYCP family connection found, and achieve the effect of improving low phosphorus tolerance and high tolerance

Active Publication Date: 2016-04-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
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Problems solved by technology

But so far no link between the CYCP family in Arabidopsis and the response to phosphorus starvation signals has been found

Method used

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  • Rice cyclin OsCYCP4 ; 4 application and method for improving rice tolerance to low phosphate stress
  • Rice cyclin OsCYCP4 ; 4 application and method for improving rice tolerance to low phosphate stress
  • Rice cyclin OsCYCP4 ; 4 application and method for improving rice tolerance to low phosphate stress

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Embodiment

[0026]A method for improving the resistance of rice to low phosphorus stress, by knocking out the rice cell cycle gene OsCYCP4; 4, the coding gene of rice cell cycle protein OsCYCP4; The ability of low phosphorus stress is improved.

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Abstract

The present invention discloses rice cyclin OsCYCP4 ; 4 application and a method for improving rice tolerance to low phosphate stress, and OsCYCP4 ; 4 transcription level expression and OsCYCP4 ; 4 translation level expression are both induced by phosphorus starvation stress. Inhibition of overexpression of OsCYCP4; 4 gene on the growth of rice proves that the overexpression of OsCYCP4; 4 gene negatively regulates the growth of rice. Under normal culture conditions, and compared with a wild type, an OsCYCP4; 4 functional deletion mutant has no obvious phenotypic differences, but the phosphorus content of the mutant is significantly higher than that of the wild type. Under phosphorus deficiency conditions, the OsCYCP4; 4 functional deletion mutant reduces sensitivity of low-phosphorus inhibition on growth of overground parts. A guarantee for improvement of plant tolerance to low phosphorus and cultivation of new rice varieties suitable for phosphorus poor soils is provided.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to the application of rice cell cycle protein OsCYCP4;4 and a method for improving rice tolerance to low phosphorus stress. Background technique [0002] Phosphorus is one of the macroelements necessary for all life. Phosphorus not only constitutes important macromolecules in cells including DNA, RNA, ATP and phospholipids, but also participates in regulating important physiological and biochemical processes such as signal transduction, energy metabolism and photosynthesis (AbelS (2011) Phosphatessensinginrootdevelopment.CurrOpinPlantBiol14 :303-309). With the continuous improvement of the research level of molecular biology, we have a certain understanding of the complex physiological and biochemical processes of phosphorus uptake and metabolism in plants. Genes involved in response to low phosphorus stress in plants are continuously being discovered, including ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8271
Inventor 易可可邓敏娟王芳
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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