An instrument device for cryopreservation of biological materials

Abstract

The present invention discloses an instrument apparatus for low-temperature storage of a biological material. The instrument apparatus at least comprises a low-temperature protection agent storage tank, a protection agent dilution buffer liquid storage tank, a miniature electromagnetic valve, a miniature electromagnetic valve, a constant flow pump, a programmable controller, a PC computer, a data acquisition card, an electromagnetic feedback density sensing system, a temperature measurement sensor, sterile filters, a container cover, a biological material storage container, a stainless steel container housing, a temperature control device, a small peristaltic pump, and a valve. According to the present invention, with the instrument apparatus, the addition of the low-temperature protection agent can be strictly controlled, the whole cooling process can be controlled, the low-temperature protection agent is synchronously subjected to elution during the rewarming control, the toxicity of the low-temperature protection agent can be minimized, the tissue cells can achieve the low temperature state within the specified time range under the room temperature state, and the specimen can recover to the room temperature state from the low temperature state within the specified time if necessary.

Description

technical field [0001] The invention relates to the technical field of instruments and containers for low-temperature preservation of biological materials, in particular to an instrument and device for low-temperature preservation of biological materials. The instrument device can simultaneously control the cooling process of the solution and the material when the cryoprotectant solution is added; it can also simultaneously elute the cryoprotectant when controlling the reheating of the solution and the material. Background technique [0002] With the development of science and technology, cryopreservation technology of biological materials (proteins, nucleic acids, cells, organelles, tissues and organs, etc.) has been widely used in the fields of pharmaceuticals, bioengineering, tissue engineering, regenerative medicine and clinical medicine. Application, cryopreservation of biological materials, especially for the preservation of large volume and complex structure of tissue...

AI Technical Summary

Problems solved by technology

As early as 1965, Farrant proposed another solution, that is, based on the characteristic that the toxicity of the cryoprotectant decreases with temperature, the concentration of the cryoprotectant is increased while cooling down, and according to the principle of "liquidus line tracking", it is determined that the temperature of the cryoprotectant at different temperatures When the temperature drops to a certain temperature, add enough cryoprotectant to avoid ice nucleation, but determine the required cryoprotectant concentration at different temperatures, and artificially reduce the temperature to different temperatures. Adding additional cryoprotectant multiple times is time-consuming, difficult to control, and even more difficult to standardize the method

It is more difficult to gradually reduce the concentration of cryoprotectant during the rewarming process without causing osmotic stress and solution freezing, and the existing temperature control settings work by controlling the ambient temperature. In addition, it is easy to cause contamination of biological materials. To solve the above problems, we have invented an instrument for low temperature storage of biological materials.

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