Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

NKT (Natural Killer T) cell preparation prepared by sensitization by using tumor cells and simulating factors and preparation method of NKT cell preparation

A technology of NKT cells and stimulating factors, which is applied in the field of biomedicine, can solve the problems of poor killing activity and insignificant amplification effect, and achieve the effect of enhancing the amplification effect and improving the killing effect

Inactive Publication Date: 2016-05-04
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, IL-2 is mainly used to promote the proliferation of NKT cells, the expansion effect is not significant, and the killing activity is relatively poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • NKT (Natural Killer T) cell preparation prepared by sensitization by using tumor cells and simulating factors and preparation method of NKT cell preparation
  • NKT (Natural Killer T) cell preparation prepared by sensitization by using tumor cells and simulating factors and preparation method of NKT cell preparation
  • NKT (Natural Killer T) cell preparation prepared by sensitization by using tumor cells and simulating factors and preparation method of NKT cell preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This embodiment provides a method for preparing NKT cell preparations sensitized with tumor cells and stimulating factors, comprising the following steps:

[0028] 1) Collect 20 mL of the patient's own peripheral blood, and collect the plasma after centrifugation;

[0029] 2) Dilute the blood cells in the lower layer with normal saline, then add the diluted solution into a sepmate centrifuge tube with Ficoll separation solution, centrifuge at 300g for 10min, and the volume ratio of blood cells to normal saline is 1:2;

[0030] 3) Pour out the supernatant liquid after centrifugation in step 2) from the centrifuge tube, then add 15 mL of RPMI1640 medium to resuspend the cells, and centrifuge at 300 g for 10 min;

[0031] 4) Add X-VIVO-15 medium for culture, the cell density is 5×10 5 / mL; at the same time, add 100U / mL of IL-15, 100U / mL of IL-21, 50μg / mL of OKT-3, 100U / mL of IL-2 to the culture medium, and observe the morphology of NKT cells every day. , the morphology o...

Embodiment 2

[0041] This embodiment provides a method for preparing NKT cell preparations sensitized with tumor cells and stimulating factors, comprising the following steps:

[0042] 1) Collect 20 mL of the patient's own peripheral blood, and collect the plasma after centrifugation;

[0043] 2) Dilute the blood cells in the lower layer with normal saline, then add the diluted solution into a sepmate centrifuge tube with Ficoll separation solution, centrifuge at 400g for 15min, and the volume ratio of blood cells to normal saline is 1:1;

[0044] 3) Pour out the supernatant liquid after centrifugation in step 2) from the centrifuge tube, then add 20 mL of RPMI1640 medium to resuspend the cells, and centrifuge at 400 g for 15 min;

[0045] 4) Add X-VIVO-15 medium for culture, the cell density is 8×10 5 / mL; at the same time, add 500U / mL IL-15, 500U / mL IL-21, 300μg / mL OKT-3, 500U / mL IL-2 to the culture medium, and observe the morphology of NKT cells every day;

[0046] 5) Count the cells o...

Embodiment 3

[0052] This embodiment provides a method for preparing NKT cell preparations sensitized with tumor cells and stimulating factors, comprising the following steps:

[0053] 1) Collect 20 mL of the patient's own peripheral blood, and collect the plasma after centrifugation;

[0054] 2) Dilute the blood cells in the lower layer with normal saline, then add the diluted solution into a sepmate centrifuge tube with Ficoll separation solution, centrifuge at 500g for 20min, and the volume ratio of blood cells to normal saline is 2:1;

[0055] 3) Pour out the supernatant liquid after centrifugation in step 2) from the centrifuge tube, then add 25 mL of RPMI1640 medium to resuspend the cells, and centrifuge at 500 g for 20 min;

[0056] 4) Add X-VIVO-15 medium for culture, the cell density is 10×10 5 / mL; at the same time, add 1000U / mL IL-15, 1000U / mL IL-21, 500μg / mL OKT-3, 1000U / mL IL-2 to the medium, and observe the morphology of NKT cells every day;

[0057] 5) Count the cells once ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biomedicines, in particular to an NKT (Natural Killer T) cell preparation prepared by sensitization by using tumor cells and simulating factors and a preparation method of the NKT cell preparation. The NKT cell preparation is high in killing activity and can effectively kill remaining cancer cells, eliminate recurrence and metastasis factors, increase the curability, prolong the survival time of patients and improve the life quality by applying an immunotherapy. In the preparation process of the preparation, NKT cells are jointly cultured by adopting IL-2, IL-15 and IL-21 and sensitized by adopting the tumor cells and the stimulating factors, so that the expansion effects of the NKT cells are enhanced, and the killing activity of the NKT cell preparation is also greatly enhanced.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an NKT cell preparation sensitized with tumor cells and stimulating factors and a preparation method thereof. Background technique [0002] Tumor cell immunotherapy is an emerging tumor treatment model with significant curative effect, and it is a new treatment method for autoimmune anti-cancer. It is a method of using biotechnology and biological agents to culture and amplify immune cells collected from patients in vitro and then reinfuse them back into the patient's body to stimulate and enhance the body's own immune function, so as to achieve the purpose of treating tumors, experimental and clinical All suggest that the immune system of the body has the effect of eliminating cancer. After the primary cancer is surgically removed or the local cancer is ablated by minimally invasive surgery such as argon-fluorine knife, immunotherapy can kill the remaining cancer cells and e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P35/00C12N5/0783
CPCA61K39/0011A61K2039/5158C12N5/0646C12N2501/25C12N2501/998
Inventor 王一飞陈海佳葛啸虎罗二梅
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com