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Pseudomonas putida and application thereof to promotion of growth of cercidiphyllum japonicum

A technology of Pseudomonas putida, fragrant tree, applied in the application, plant growth regulator, plant growth regulator and other directions, can solve the problem of no growth-promoting bacteria of fragrant tree, achieve excellent strain resources, promote growth and development , the effect of strong growth hormone secretion ability

Active Publication Date: 2016-05-04
长治学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no reports on the growth-promoting bacteria of Lianxiang tree at home and abroad

Method used

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  • Pseudomonas putida and application thereof to promotion of growth of cercidiphyllum japonicum
  • Pseudomonas putida and application thereof to promotion of growth of cercidiphyllum japonicum
  • Pseudomonas putida and application thereof to promotion of growth of cercidiphyllum japonicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of the phosphorus-dissolving ability of LWPZF liquid.

[0027] NBRIP medium: glucose 10g, Ca 3 (PO 4 ) 2 5.0g, MgCl 2 5g, MgSO 4 ·7H 2 O0.25g, KCl0.2g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH7.0.

[0028] Inoculate the twice-activated LWPZF strain into NB medium (3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, 1000 mL of distilled water, pH 7.2-7.4), and culture at 30°C for 18-24 hours to make seed liquid. Take 0.5mL seed solution and inoculate it into a 100mL Erlenmeyer flask containing 50mL NBRIP culture solution, and use NBRIP medium inoculated with 0.5mL blank seed solution as a control. Each treatment has 3 replicates, 30°C, 180r / min shaking culture for 4 days. The fermented liquid was centrifuged at 10000r / min for 10min at 4°C, and the molybdenum-antimony anti-colorimetric method was used to measure the soluble phosphorus content in the fermented liquid ( figure 1 ).

[0029] From figure 1 It can be ...

Embodiment 2

[0029] From figure 1 It can be seen from the figure that the soluble phosphorus content in the fermentation liquid inoculated with LWPZF strain was 742.04 mg·L -1 , is the control (32.81mg·L -1 ) 22.6 times. In summary, the LWPZF strain has a strong ability to degrade calcium phosphate. Example 2: Determination of the ability of LWPZF to produce auxin.

[0030] King'sB medium: peptone 20g, MgSO 4 ·7H 2 O1.5g, K 2 HPO 4 1.5g, glycerol 10mL, agar 15g, after adjusting the pH value to 7.0, add to 1000mL.

[0031] S1 colorimetric solution: accurately weigh 12g FeCl 3 Dissolve in 300mL of deionized water, then slowly add 429.7mL of concentrated sulfuric acid, and dilute to 1L after cooling.

[0032] Prepare IAA standard solutions with concentrations of 1, 4, 6, 8, 10, 12, 14, 16, 18, and 20 mg / L, and mix them with S1 reagent at a volume ratio of 1:1, place them at room temperature in the dark for 30 minutes, and then Determine the OD of each concentration 530nm (The 1:1 m...

Embodiment 3

[0035] Example 3: Determination of the ability of LWPZF to promote growth of cucumber seeds.

[0036] The cucumber seeds were treated with 10% hydrogen peroxide for 20 minutes, washed 5-6 times with sterile water, and dried for later use.

[0037] The LWPZF strain was inoculated in NB liquid medium, and cultured on a shaker at 28°C for 24-36 hours, so that the bacterial concentration reached 10 9 cfu / mL. The bacterial suspension was serially diluted to 10 -7 , divide the bottom of the sterile petri dish into 8 equal parts with a marker pen, mark CK, -1, -2, -3, -4, -5, -6, -7 in turn, place 4~ 6 pieces of filter paper, put sterile absorbent cotton in the middle, put the processed cucumber seeds on the filter paper, drop 100 μl of LWPZF bacterial suspension corresponding to the dilution in the center of the filter paper in each area of ​​the bottom of the dish, drop in the CK area, etc. Amount of sterile water was used as a control. 28°C, cultivated under light, and observe...

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PUM

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Abstract

The invention discloses pseudomonas putida which is classified and named as pseudomonas putida, the strain number is LWPZF, the pseudomonas putida is preserved in China Center for Type Culture Collection, the preservation serial number is CCTCC NO: M 2016008, and the preservation data is Jan 5th, 2016. The invention further discloses application of the pseudomonas putida to promotion of growth of cercidiphyllum japonicum. The LWPZF strain has high inorganic phosphorus decomposition capacity and growth hormone secretion capacity; germination of cucumber seeds can be obviously promoted; the LWPZF fungicide is inoculated with cercidiphyllum japonicum seedlings; it is proved by results that the fungicide can obviously promote growth of cercidiphyllum japonicum seedlings; meanwhile, the culture has a certain antagonistic effect on fusarium oxysporum, rhizoctonia solani, rhizoctonia, rice blast pathogen and other phytopathogen; therefore an excellent strain resource is provided for development of a biofertilizer special for cercidiphyllum japonicum.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a pseudomonas putida and its application in promoting the growth of japonica. Background technique [0002] Cercidiphyllum japonicum Sieb.EtZucc. is a deciduous tree belonging to Cercidiphyllum japonicum Sieb.EtZucc. Lianxiang tree remnants are mainly distributed in the subtropical and warm temperate regions of China. This species is a relic single family plant of the ancient tropical plants of the Tertiary Period, and it is an ancient and primitive woody plant. Dioecious, fruit less, its seedlings are vulnerable to natural conditions such as disease and insect pests, rainstorms, so it is difficult to grow under natural conditions, and there are very few young trees under the forest. Lianxiang tree resources are scarce and are on the verge of extinction. Therefore, it is included in the "List of Rare and Endanger...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/40
CPCA01N63/00C12N1/205C12R2001/40
Inventor 任嘉红张桂萍晋婷婷白凤麟彭卿王莹
Owner 长治学院
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