Preparation method of antibacterial artificial seeds of orychophragmus violaceus

The technology of an artificial seed and its production method is applied in the field of biotechnology and agricultural engineering, which can solve the problems of antisepsis and antibacterial hindering the large-scale promotion of artificial seed technology, and achieve the goal of improving germination rate and germination speed, good antibacterial effect, and small plant toxicity Effect

Active Publication Date: 2016-05-11
无锡智慧兴宜信息技术有限公司
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Problems solved by technology

[0004] The problem of antisepsis and antibacterial has always been a major problem hindering the large-scale promotion of artificial seed technology. Commonly used fungicides such as carbendazim, penicillin, and cephalomycin all have certain negative effects on plants, and adding them alone can only resist several fungi Pollution...

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  • Preparation method of antibacterial artificial seeds of orychophragmus violaceus
  • Preparation method of antibacterial artificial seeds of orychophragmus violaceus

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Embodiment Construction

[0021] The technical solution of the present invention will be further described in detail below in conjunction with the embodiments.

[0022] In an embodiment of the present invention, a kind of antibacterial Zhuge vegetable artificial seed production method comprises the following steps:

[0023] Step 1, bud induction of Cauliflower: Take Cauliflower seedlings, wash them with sterile water for 3 minutes, cut out the axillary buds and terminal buds, complete disinfection on the ultra-clean workbench, and inoculate aseptically on the bud induction medium for bud induction culture; Bud induction medium includes: MS medium, 2mg / L6-BA (6-benzylaminoadenine), 0.2mg / LNAA (α-naphthaleneacetic acid), 3% sucrose, 0.65% agar; the light intensity of the bud induction process 2000~3000Lux fluorescent lamp for 12 hours;

[0024] Step 2, bud propagation culture of Zhuge Cai: take the Zhuge Cai tissue culture seedlings cultured in step 1 bud induction, cut out the young shoots, and transfe...

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Abstract

The invention discloses a preparation method of antibacterial artificial seeds of orychophragmus violaceus, and belongs to the field of biotechnology and agricultural engineering. The method comprises the steps of bud inducing of orychophragmus violaceus, bud propagating and cultivating, bud embedding and artificial seed capsule preparing, and sodium alginate hydrosol with the mass concentration of nano-titanium dioxide of 7% is adopted as an embedding solution used in the bud embedding and artificial seed capsule preparing processes. The artificial seeds prepared through the preparation method are high in rooting and budding rate and low in contamination rate in a bacterial environment; compared with artificial seeds added with a traditional bactericide, the prepared artificial seeds have the advantages of being better in antibacterial effect, higher in budding rate and lower in toxic action on explants, and the artificial seed technology of orychophragmus violaceus is further pushed to production and application.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural engineering, and in particular relates to a method for preparing an antibacterial artificial seed of Zhuge Cai. Background technique [0002] Zhuge Cai belongs to the cruciferous one or two-year herbaceous plant, which is widely distributed in Northeast my country and North China. It has strong adaptability, cold resistance, drought tolerance, shade tolerance, and few pests and diseases. The flowers are purple or white, and their life history is from September to June of the next year. Even in the cold winter, they can still keep green and cover the ground well. It is a rare flower in early spring and green in winter in the northern region. The ground cover plant is very popular among people. In the fatty acid composition of the seeds of Zhugecai, the content of erucic acid and linolenic acid is low, and the content of palmitic acid, oleic acid and linoleic acid is 89.8% of the tota...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 吴沿友谷睿智邢德科于睿胡林生吴沿胜黎明鸿姚香萍
Owner 无锡智慧兴宜信息技术有限公司
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