Real-time fluorescence rpa kit, test strip rpa kit and application thereof for rapid detection of sheep pox virus

Abstract

The invention discloses a real-time fluorescent RPA kit for rapidly detecting capripoxvirus and application thereof, and further discloses a test strip RPA kit for rapidly detecting the capripoxvirus and application thereof. The two kits comprise primers shown as SEQ ID NO.1 and SEQ ID NO.2 respectively and respective probes, and modified bases and detection platforms of the primers and the probes are different although the primers and the probes aim at a same target sequence. Experiments prove that the sensibility of the real-time fluorescent RPA kit and the sensibility of the test strip RPA kit are both 102 copies per response, and both of the two kits only can specially detect a sheep pox virus and a goat pox virus. A capripoxvirus suspected sample is detected by the two kits separately, and results show that the detection results of the two kits both have the very high conformity with that of qPCR. Therefore, the two kits both can rapidly, efficiently and sensitively detect the sheep pox virus or the goat pox virus, and an effective technological means is supplied to differentiation and diagnosis of the sheep pox virus or the goat pox virus.

Description

Technical field [0001] The present invention relates to a kit for detecting sheeppox virus and its use, in particular to a real-time fluorescent RPA kit and test strip RPA kit for rapid detection of sheeppox virus based on RPA reaction, and also relates to both The application in rapid detection of sheeppox virus belongs to the field of preventive veterinary inspection. Background technique [0002] It has been thirty years since the birth of PCR technology. From classic PCR, real-time quantitative PCR to current digital PCR, this technology is constantly evolving but never fades out of our field of vision. However, this technology requires special and expensive thermal cycling equipment and skilled operators, which is time-consuming and labor-intensive, and is difficult to use in on-site diagnosis and poor experimental conditions. In developing country laboratories, due to the limitations of the basic equipment and technology required for PCR implementation, most developing co...

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Problems solved by technology

[0010] The present invention is aimed at the GPCR gene of sheeppox virus, establishes and evaluates the RPA (real-timeRPA) detection kit based on fluorescent probe and the LFS RPA detection kit based on test strip to realize the purpose of fast detection sheeppox virus, according to As far as we know, no such kit has been established at home and abroad for the detection of sheep pox virus

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