Establishing method for rabbit tongue carcinoma model

Abstract

The invention provides an establishing method for a rabbit tongue carcinoma model. The method comprises the following steps: establishing rabbit tongue squamous carcinoma cell lines; inoculating the rabbit tongue squamous carcinoma cells under skin of a nude mouse and culturing the nude mouse; taking cultured nude mouse subcutaneous tumor tissue, and inoculating the subcutaneous tumor tissue on a rabbit tongue, to establish a rabbit tongue carcinoma model; based on the rabbit tongue carcinoma model, establishing a high lymph gland metastatic rabbit tongue carcinoma animal model. Establishing a tongue carcinoma animal model with high lymph gland metastatic potential completely re-represents clinical characteristics of tongue carcinoma and lymphatic metastasis biological characteristics, and provides better experimental basis for prevention and treatment of tongue carcinoma. The method can be used for sensitivity evaluation of anti-tumor chemotherapeutics, and can also be used for research of radiotherapy, endocrinotherapy, immunological therapy, molecular targeting treatment, and other new treatment means.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for establishing an animal model of rabbit tongue cancer. Background technique [0002] In recent years, with the continuous development of basic and clinical medical research, the diagnosis and treatment of oral and maxillofacial squamous cell carcinoma (OSCC) have been significantly improved. The 5-year survival rate has reached about 64%, but more than 1 / 3 of the patients died of local tumor recurrence and / or distant metastasis within 5 years[1-2]. About 25% of patients with early stage (I, II) oral cancer cannot be cured, while for advanced stage (III, IV) patients, this figure is even higher [3]. Therefore, clarifying the pathogenesis of oral and maxillofacial malignant tumors, comprehensively improving the cure rate of the disease and effectively preventing its occurrence and development have become urgent tasks for the Department of Oral and Maxillofacial-Head and Neck Onc...

AI Technical Summary

Problems solved by technology

In addition, the average lifespan of nude mice is short, which cannot meet the requirements such as the screening of anticancer drugs and the selection of the best chemotherapy regimen, the mechanism of curative effect of combined radiotherapy and chemotherapy in the treatment of maxillofacial metastatic cancer, and the targeting of related drugs in imaging diagnosis and interventional radiotherapy. distribution studies, etc.

Nude mice are too small for local treatments such as hyperthermia, radiotherapy, and interventional therapy

Obviously, nude mouse oral cancer models are difficult to meet the needs of basic and clinical research

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