Method for making novel kidney disease model

Abstract

The invention discloses a method for making a novel kidney disease model. In particular, the renal artery of a mouse is occluded for 60 minutes, and the mouse can survive; renal pathological examination is performed on the 21st day, and chronic renal injury pathological changes such as diffuse fibrosis can be found. Compared with the prior art, the method has the advantages that after successful establishment of an animal model, an effective tool is provided for possible deep researches of the mechanism and intervention of AKI to CKD transformation in the field of kidney diseases, the modeling animal mortality is lowered greatly, and chronic renal injuries are formed effectively; the method has popularization and application values.

Description

technical field [0001] The invention relates to the construction of a kidney disease model, in particular to a method for making a novel kidney disease model. Background technique [0002] With the development of social economy and the change of people's lifestyle, chronic kidney disease (CKD) has become an epidemic trend, and the incidence rate in the population is as high as 10%. Given that people's understanding of the pathogenesis of CKD is not deep enough, and there is a lack of satisfactory clinical intervention methods, most patients eventually enter the stage of end-stage renal disease, requiring blood purification and kidney transplantation, which brings a heavy burden to families and society. Among the causes of CKD, the progressive progression after acute kidney injury (AKI) is considered to be one of the important causes of CKD. Therefore, in-depth study and understanding of the molecular pathological mechanism of the progression from AKI to CKD and the developm...

AI Technical Summary

Problems solved by technology

However, due to the high mortality rate of these traditional AKI models (the mortality rate of long-term observation can even reach more than 80-90%), the surviving animals may have died due to the anti-injury effect related to their genetic background (most of the kidneys with severe kidney damage , those with mild kidney damage can survive, which may be related to genetic differences), and their pathological damage is mild, unable to present typical CKD pathological manifestations (extensive fibrosis and renal tubular atrophy, etc.)

It is the lack of widely used and recognized animal models for the progression of AKI to CKD that prevents rapid progress in research in this field and greatly limits the development of clinical interventions