Antibacterial products based on gold nanoparticles and its preparation method and application

A gold nanoparticle and product technology, applied in the field of antibacterial products based on gold nanoparticles, can solve the problems of harsh preparation conditions, poor adhesion, unfavorable production of antibacterial products, etc., to save cleaning steps, stable properties, and prevent bacterial nosocomial infection Effect

Active Publication Date: 2020-04-07
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, people have been worrying about the problem that silver nanoparticles are also toxic.
Among these methods, some have poor adhesion between nanoparticles and solid surfaces (such as direct coating), some require harsh preparation conditions (such as high temperature or negative pressure), and some require additional chemical reagents. and reaction time (such as chemical modification), which are not conducive to the industrial scale production of antibacterial articles with nanoparticles

Method used

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  • Antibacterial products based on gold nanoparticles and its preparation method and application
  • Antibacterial products based on gold nanoparticles and its preparation method and application
  • Antibacterial products based on gold nanoparticles and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example is used to illustrate the antibacterial article based on gold nanoparticles and its preparation of the present invention.

[0045] The base materials used in this embodiment are glass tubes (made of glass, purchased from Beijing Kelong Company), syringes (made of polypropylene, purchased from Shanghai Zhiyu Medical Instrument Co., Ltd.) and catheters (made of silica gel, purchased from Shanghai Zhiyu Medical Instrument Co., Ltd.). from Guangzhou Weili Medical Devices Co., Ltd).

[0046] A. The preparation steps of the antibacterial glass tube based on gold nanoparticles of the present invention are as follows:

[0047] (1) Auric acid chloride, DAPT and surfactant are mixed to obtain a mixed solution. Wherein, the molar ratio of DAPT and chloroauric acid in the mixed solution is 1:1. The surfactant is Tween-80, and its volume content in the mixed solution is 0.1%.

[0048] (2) Adding a reducing agent to the mixed solution in step (1) for reduction to obta...

Embodiment 2

[0065] This example is used to illustrate the antibacterial article based on gold nanoparticles and its preparation of the present invention.

[0066] The substrate used in this example is a 96-well plate (the material is polystyrene, purchased from Corning Company).

[0067]The preparation steps of the antibacterial 96-orifice plate based on gold nanoparticles of the present invention are as follows:

[0068] (1) Auric acid chloride, DAPT and surfactant are mixed to obtain a mixed solution. Wherein, the molar ratio of DAPT and chloroauric acid in the mixed solution is 1:1. The surfactant is Tween-80, and its volume content in the mixed solution is 0.1%.

[0069] (2) Adding a reducing agent to the mixed solution in step (1) for reduction to obtain a DAPT-modified gold nanoparticle solution. Among them, the reducing agent is NaBH 4 , and its molar ratio with chloroauric acid is 3:1. The time of the reduction reaction is 1h, the reaction temperature is 0°C, the concentratio...

Embodiment 3

[0078] This example is used to illustrate the physical properties of the antibacterial layer of the antibacterial product of the present invention.

[0079] Use an inductively coupled plasma optical emission spectrometer (ICP-OES) (instrument model Optima5300DV, purchased from U.S. Pekin-Elmer Company) to measure the gold element content (μg / cm2) of the antibacterial 96-well plate prepared in Example 2 and the contrast 96-well plate 2 ).

[0080] Wherein, the comparison 96-well plate is prepared by the following steps: take the common 96-well plate with the same specification as the antibacterial 96-well plate of Example 2, and directly immerse it in the DAPT-modified gold nanoparticle solution of Example 2 for 12h, After taking it out, it was washed three times with PBS, then ultrasonically cleaned for 30 min, and dried.

[0081] Measurement results such as Figure 4 shown. exist Figure 4 In , the columns filled with slashes from the upper left to the lower right indicat...

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Abstract

The invention provides an antibacterial product based on gold nanoparticles, the antibacterial product includes an antibacterial layer and a substrate, the antibacterial layer includes gold nanoparticles modified by DAPT, the surface of the substrate is negatively charged, the antibacterial layer The antibacterial product is combined on the surface of the base material through electrostatic interaction, and the negative charge on the surface of the base material is obtained by treating the base material in a plasma cleaning device. The preparation method and application of the antibacterial product are also provided.

Description

technical field [0001] The invention relates to an antibacterial product, in particular to an antibacterial product based on gold nanoparticles, a preparation method and application of the antibacterial product. Background technique [0002] Bacterial infection has been a major disease threatening human health since ancient times. In the 1940s, humans invented antibiotics to fight against bacterial infection, which greatly reduced the medical accidents caused by bacterial infection. However, with the abuse of antibiotics in recent years, some new types of multidrug-resistant bacteria have gradually emerged. These bacteria, which cannot be killed by current commercial antibiotics, are known as superbugs. With the emergence of superbugs, bacterial infection has once again become a major threat to human health. [0003] As one of the places where antibiotics are used most frequently, hospitals naturally produce many superbugs. In recent years, many patients in major hospital...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61L29/10A61L29/14A61L31/08A61L31/14C12M3/00C03C17/00B22F1/00B22F9/24
CPCA61L29/106A61L29/14A61L31/088A61L31/14C12M23/12C12M23/20C12M37/00C03C17/006C03C17/009B22F9/24C03C2218/111C03C2217/70C03C2217/40A61L2300/224A61L2300/404B22F1/054
Inventor 蒋兴宇郑问枢
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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