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A kind of opb oligopeptide with anticancer activity and its expression vector and application

An anti-cancer activity, expression vector technology, applied in medical preparations containing active ingredients, introduction of foreign genetic materials, peptide sources using vectors, etc., can solve the problems of difficult separation and purification, difficult to synthesize polypeptide molecules, etc. , low cost and high output

Active Publication Date: 2019-06-07
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Problems in the prior art: (1) Bacterial system expression products may contain endotoxin, but this shortcoming can be solved by using a method for removing endotoxin
(2) The expression system based on DNA plasmid is difficult to synthesize too small polypeptide molecules, because it is difficult to separate and purify

Method used

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  • A kind of opb oligopeptide with anticancer activity and its expression vector and application
  • A kind of opb oligopeptide with anticancer activity and its expression vector and application
  • A kind of opb oligopeptide with anticancer activity and its expression vector and application

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Experimental program
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Effect test

specific Embodiment approach 1

[0033]Specific embodiment 1: An OPB oligopeptide with anticancer activity according to this embodiment, its sequence is Gly-Asn-Lys-Lys-Trp-Glu-Gln-Lys-Gln-Val-Gln-Ile-Lys-Thr -Leu-Glu-Gly-Glu-Phe-Ser-Val-Thr-Met-Trp-Ser-Ser.

specific Embodiment approach 2

[0034] Embodiment 2: An OPB oligopeptide with anticancer activity according to this embodiment, the oligopeptide or any part of the oligopeptide is used to prepare a drug for treating cancer.

specific Embodiment approach 3

[0035] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the oligopeptide or a part of the oligopeptide is used to prepare a drug for treating cancer. Others are the same as in the second embodiment.

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Abstract

The invention relates to an anticancer active oligopeptide and an expression vector and application thereof, in particular to anticancer active OPB (oligopeptidase B) oligopeptide and an expression vector and application thereof. A sequence of the oligopeptide is Gly-Asn-Lys-Lys-Trp-Glu-Gln-Lys-Gln-Val-Gln-Ile-Lys-Thr-Leu-Glu-Gly-Glu-Phe-Ser-Val-Thr-Met-Trp-Ser-Ser. The oligopeptide is used to prepare anticancer drugs. The expression vector pMBP-2xOPB is constructed. A peptide fragment synthesized by using the OPB sequence can inhibit the growth and proliferation of tumor cells, and it is shown that the OPB-based peptide fragment and its derivatives are potential drugs for treating cancers.

Description

technical field [0001] The invention relates to an oligopeptide with anticancer activity, its expression carrier and application. Background technique [0002] Yin Yang 1 (YY1) is a multifunctional protein with tumor cell growth-promoting activity. YY1 has an OPB (Oncoprotein Binding) region (such as figure 1 shown), can bind to multiple proteins (oncoproteins) that promote cancer initiation and progression, including AKT, Mdm2, E1A and Ezh2. [0003] The occurrence and development of cancer is accompanied by genetic and epigenetic changes. Epigenetics are those heritable traits that do not involve changes in DNA sequence when cells divide. Epigenetics can coordinate the role of genetic information in the process of differentiation and development. Defects in these regulations lead to various diseases in humans, including cancer. YY1 is involved in many epigenetic regulatory mechanisms in tumorigenesis. So far, most studies have shown that the biological function of YY...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/70C12N15/66A61K38/17A61P35/00
CPCA61K38/00C07K14/47C12N15/66C12N15/70
Inventor 隋广超
Owner NORTHEAST FORESTRY UNIVERSITY