Preparation method of Brevibacillus brevis wettable powder
A Bacillus brevis wettable powder technology, applied in the field of Bacillus brevis wettable powder and its preparation, can solve the problems of short control effect, high suspension rate, and short wetting time, so as to reduce the probability of germination, The effect of reducing exposure
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Embodiment 1
[0033] A Bacillus brevis wettable powder consists of the following weight components: 60 parts of Bacillus brevis fermentation broth; filler: 25 parts of white carbon black; wetting agent: 3 parts of sodium lauryl sulfate; dispersant: T-type wood Spore germination agent: 0.2 parts of inosine, 0.4 parts of fructose; UV protection agent: 1.5 parts of lecithin, 0.8 parts of β-dextrin; drying protection agent: 3 parts of maltose, 0.6 parts of sucrose.
[0034] A preparation method of bacillus brevis wettable powder, comprising the following steps:
[0035] 1. Preparation of Bacillus brevis fermentation broth
[0036] Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 30°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 30°C for 24 hours. The seeds are inoculated into the bacillus liquid culture medium in a 5L large triangular flask ...
Embodiment 2
[0044] Control effect of Bacillus brevis wettable powder on watermelon wilt in field
[0045] The test location: a farm in Xiaoshan District, Hangzhou City, Zhejiang Province, where watermelons are grown in greenhouses all year round. Due to the perennial dryness, the diseases are relatively serious, especially Fusarium wilt;
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