Preparation method of Brevibacillus brevis wettable powder

A Bacillus brevis wettable powder technology, applied in the field of Bacillus brevis wettable powder and its preparation, can solve the problems of short control effect, high suspension rate, and short wetting time, so as to reduce the probability of germination, The effect of reducing exposure

Active Publication Date: 2016-06-08
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is also no report on the research of adding a spore germination agent in the Bacillus brevis wettable powder
[0012] In addition, due to the compatibility of different bacillus with wetting agents, dispersants and other additives, the carrier has different adsorption properties to various bacillus, and the existing formula or preparation process of bacillus wettable powder cannot be used. In the preparation of Bacillus brevis wettable powder containing spore germination agent; the adsorption amount of effective bacteria in the wettable powder, the survival rate, the control effect on harmful bacteria and the s

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A Bacillus brevis wettable powder consists of the following weight components: 60 parts of Bacillus brevis fermentation broth; filler: 25 parts of white carbon black; wetting agent: 3 parts of sodium lauryl sulfate; dispersant: T-type wood Spore germination agent: 0.2 parts of inosine, 0.4 parts of fructose; UV protection agent: 1.5 parts of lecithin, 0.8 parts of β-dextrin; drying protection agent: 3 parts of maltose, 0.6 parts of sucrose.

[0034] A preparation method of bacillus brevis wettable powder, comprising the following steps:

[0035] 1. Preparation of Bacillus brevis fermentation broth

[0036] Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 30°C for 48 hours. Pick a single colony under the plate and inoculate it into 50 ml of LB medium, and culture it in an incubator with shaking at 30°C for 24 hours. The seeds are inoculated into the bacillus liquid culture medium in a 5L large triangular flask ...

Embodiment 2

[0044] Control effect of Bacillus brevis wettable powder on watermelon wilt in field

[0045] The test location: a farm in Xiaoshan District, Hangzhou City, Zhejiang Province, where watermelons are grown in greenhouses all year round. Due to the perennial dryness, the diseases are relatively serious, especially Fusarium wilt;

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PUM

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Abstract

The invention relates to the fields of biopesticide and microbial fertilizer, and specifically discloses a Brevibacillus brevis wettable powder containing a spore germinator, and a preparation method thereof.. The wettable powder consists of the following components by weight: 40-70 parts of a Brevibacillus brevis fermentation broth, 20-30 parts of a white carbon black filler, 1-4 parts of a wetting agent sodium lauryl sulfate, 0.2-1 part of a dispersant of T lignin, a spore germinator containing 0.1-0.5 part of inosine and 0.1-0.5 part of fructose, a UV protective agent containing 0.5-2 parts of lecithin and 0.2-1 part of beta-dextrin, and a drying protecting agent containing 1-4 parts of maltose and 0.2-1 part of sucrose. After application of the Brevibacillus brevis wettable powder into the field, the spores have high germination speed and high germination rate; and the fertilizer efficiency and control effect on plant disease of the Brevibacillus brevis wettable powder can be significantly improved, the amount of application and the production costs can be reduced.

Description

technical field [0001] The invention relates to the technical field of biological pesticides and microbial fertilizers, in particular to a Bacillus brevis wettable powder containing a brevis spore germination agent and a preparation method thereof. Background technique [0002] my country uses a large amount of chemical pesticide preparations and fertilizers every year, which can easily cause excessive pesticide residues and bio-enrichment, which not only pollutes the ecological environment, but also affects human health. However, biopesticide has few side effects and is harmless to humans. It is a kind of green pesticide with broad prospects and has increasingly become a trend in the development of global pesticides. [0003] Bacillus is currently the most studied microorganism in biological pesticides and biological fertilizers. It has broad-spectrum antibacterial activity and strong resistance to stress. Its vegetative cells can reproduce and colonize on the surface of fu...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/14A01P3/00
CPCA01N25/14A01N63/10A01N2300/00
Inventor 胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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