Screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber culture

A technology of amylolytic spores and bacillus, which is applied in the fields of application, bacteria, and fish farming, and can solve problems such as the deterioration of the farming environment, economic losses in the sea cucumber farming industry, and food safety hazards.

Active Publication Date: 2019-07-23
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the deterioration of the breeding environment and the occurrence of diseases caused by high-density farming and irregular operations have brought huge economic losses to the sea cucumber farming industry.
The traditional method of disease prevention and treatment is mainly to use antibiotics. The use of antibiotics is easy to produce drug-resistant strains, and there are residual problems in animals, which endangers food safety. Therefore, it is necessary to find alternatives to antibiotics.

Method used

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  • Screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber culture
  • Screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber culture
  • Screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber culture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Isolation, screening, identification and safety verification of strains

[0013] 1. Sample

[0014] Intestines of healthy sea cucumbers collected from Laizhou sea cucumber farm in Yantai City.

[0015] 2. Culture medium:

[0016] 1) Bacillus isolation medium: glucose 10g, calcium phosphate 5g, ammonium sulfate 0.5g, potassium chloride 0.2g, magnesium sulfate heptahydrate 0.1g, manganese sulfate 0.0001g, ferrous sulfate 0.0001g, yeast extract 0.5g, Distilled water 1000ml, pH6.8-7.2, agar 20g, sterilized at 121˚C for 30min.

[0017] 2) Improved TSB medium: tryptone soybean broth (TSB) 30g, sodium chloride 10g, distilled water 1000ml, pH7.4-7.6, add 15-20g agar to prepare solid medium, and sterilize at 121˚C 20min.

[0018] 3) Starch hydrolysis medium: beef extract 5g, peptone 10g, sodium chloride 5g, soluble starch 2g, distilled water 1000ml, pH7.2-7.4, prepare solid medium, add 15-20g agar, sterilize at 115°C for 20min.

[0019] 4) Casein hydrolysis double-layer pla...

Embodiment 2

[0035] Effects of Bacillus amyloliquefaciens ZYTC1-1 on the growth, immunity and disease resistance of sea cucumber juveniles

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Abstract

The invention relates to the technical field of functional microorganism screening and application, in particular to the screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber cultivation. A strain of Bacillus amyloliquefaciens of the present invention was deposited in the China Center for Type Culture Collection, abbreviated as CCTCC, on December 24, 2015, and its address is: Wuhan University Collection Center, Luojia Mountain, Wuchang, Wuhan City, Hubei Province; requesting depositor : Ludong University; the preservation number is CCTCC NO: M2015774, and the bacterium is named Bacillus amyloliquefaciens Bacillus amyloliquefaciens ZYTC1‑1. The bacillus amyloliquefaciens of the present invention is screened from the intestinal tract of healthy sea cucumbers. The bacterial strain has strong protease and amylase production capabilities, can improve the activity of amylase in the intestinal tract of sea cucumbers, promote the growth of sea cucumbers, and improve the immunity and immunity of sea cucumbers. Disease resistance, can be widely used in sea cucumber cultivation and aquatic feed fields.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening and application, in particular to the screening of a strain of Bacillus amyloliquefaciens and its application in sea cucumber cultivation. Background technique [0002] With the increasing demand of sea cucumber at home and abroad and the international development of aquatic product trade, the sea cucumber breeding industry has developed by leaps and bounds in recent years. However, the deterioration of the breeding environment and the occurrence of diseases caused by high-density breeding and irregular operations have brought huge economic losses to the sea cucumber farming industry. The traditional way to prevent and control diseases is mainly to use antibiotics. The use of antibiotics is easy to produce drug-resistant strains, and there are residues in animals, which endangers food safety. Therefore, it is necessary to find alternatives to antibiotics. [0003] As an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01K61/00A23K50/80A23K10/18C12R1/07
CPCA01K61/00C12N1/205C12R2001/07Y02A40/81Y02A40/818
Inventor 赵彦翠袁磊孙虎山万军利王旭静王敏陈昌
Owner LUDONG UNIVERSITY
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