Epithelium culture system

A culture system and epithelial technology, applied in the direction of culture process, tissue culture, cell culture active agent, etc., can solve the problems of cumbersome operation, low growth activity, inconvenient configuration, etc., and achieve simple configuration, reduce pollution, and promote growth activity Effect

Inactive Publication Date: 2016-06-08
ICELL SHANGHAI BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The epithelium is the basic tissue of animals composed of densely arranged epithelial cells and a very small amount of intercellular substance. It is generally connected to each other to form a membrane sheet, covering the body surface, or lining the cavity surface of the hollow organ in the body, and the body cavity According to the characteristics of function and structure, epithelial tissue can be divided into three types: covering epithelium, glandular epithelium and sensory epithelium. Widely, in the prior art, the composition of the culture medium for epithelial cell culture is relatively complex and inconvenient to configure; and in the absence of serum, the growth activity is low and the operation is cumbersome. In addition, in the existing epithelial culture process, it is easy to mix bacteria

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Embodiment Construction

[0010] The epithelial culture system of the present invention comprises an epithelial medium and an epithelial additive; the composition and concentration of the epithelial medium are: glycine 18.75mg / L, alanine 4.45mg / L, L-alanine-L-glutamine Amide 542mg / L, arginine 147.5mg / L, asparagine 7.5mg / L, aspartic acid 6.65mg / L, cysteine ​​17.55mg / L, cystine 31.3mg / L, glutamic acid 7.35mg / L, Histidine 31.48mg / L, Isoleucine 54.47mg / L, Leucine 59.05mg / L, Lysine 91.25mg / L, Methionine 17.25mg / L, Phenylalanine 35.48mg / L, proline 17.25mg / L, serine 26.25mg / L, threonine 53.45mg / L, tryptophan 9.02mg / L, tyrosine 55.8mg / L, valine 52.85mg / L, vitamin H0.0035mg / L, choline chloride 8.98mg / L, calcium pantothenate 2.24mg / L, folic acid 2.65mg / L, nicotinamide 2mg / L, vitamin B6 2.031mg / L, vitamin B20.219mg / L, vitamin B12 .17mg / L, vitamin B120.68mg / L, inositol 12.6mg / L, calcium chloride 116.6mg / L, copper sulfate 0.0013mg / L, iron nitrate 0.05mg / L, iron sulfate 0.417mg / L, Magnesium chloride 28.6mg / L, mag...

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Abstract

The invention discloses an epithelium culture system. The system comprises an epithelium culture medium and an epithelium additive, wherein the epithelium additive comprises components by concentration as follows: 25 ng/ml of a recombinant human epidermal growth factor, 100 ng/ml of hydrocortisone, 10 ug/ml of recombinant human insulin, 5 ug/ml of human total iron transferrin, 0.18 nM of adenine, 0.1 nM of cholera toxins and 20<12> M of triiodothyronine. The epithelium culture system is simple to prepare, the epithelium culture medium and the epithelium additive are matched for use, the growth activity of epithelial cells can be promoted in a 2% serum environment, the activity of fibroblast and smooth muscle cells can be partially inhibited, and parenchyma cell pollution can be reduced.

Description

technical field [0001] The invention particularly relates to an epithelial culture system, which belongs to the technical field of epithelial cell culture. Background technique [0002] The epithelium is the basic tissue of animals composed of densely arranged epithelial cells and a very small amount of intercellular substance. It is generally connected to each other to form a membrane sheet, covering the body surface, or lining the cavity surface of the hollow organ in the body, and the body cavity According to the characteristics of function and structure, epithelial tissue can be divided into three types: covering epithelium, glandular epithelium and sensory epithelium. Broadly, in the prior art, the composition of the culture medium for epithelial cell culture is relatively complex and inconvenient to configure; and in the absence of serum, the growth activity is low and the operation is cumbersome. In addition, in the existing epithelial culture process, it is easy to m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N2500/12C12N2500/14C12N2500/16C12N2500/20C12N2500/22C12N2500/24C12N2500/25C12N2500/30C12N2500/32C12N2500/34C12N2500/35C12N2500/36C12N2500/38C12N2500/40C12N2500/46C12N2501/01C12N2501/11C12N2501/999
Inventor 王悦
Owner ICELL SHANGHAI BIOSCI CO LTD
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