Method for preparing chitosan three-dimensional scaffold coated by graphene material

A three-dimensional stent, chitosan technology, applied in coatings, prostheses, medical science and other directions, can solve the problems of difficult to support tissue and organ growth, low mechanical properties, difficult to modify, etc., to achieve large-scale production, simple operation Effect

Inactive Publication Date: 2016-06-22
JILIN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

[0012] As a natural polycationic biopolysaccharide, chitosan exists in large quantities in nature and has good biocompatibility and biodegradability. The research on its use in tissue engineering is still in the ascendant, but as a bioactive scaffold, it faces Two main problems: (1) The lower mechanical properties are often difficult to support the growth of tissues and or

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  • Method for preparing chitosan three-dimensional scaffold coated by graphene material
  • Method for preparing chitosan three-dimensional scaffold coated by graphene material
  • Method for preparing chitosan three-dimensional scaffold coated by graphene material

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Embodiment

[0055] Composite materials with graphene coatings and chitosan three-dimensional scaffolds with different reduction degrees were prepared, and the specific experimental conditions were as follows:

[0056] 1) Dissolve chitosan powder in glacial acetic acid solution with a volume concentration of 2%, stir for 2 hours, make a chitosan solution with a concentration of 4% (w / v), ultrasonicate for 1 hour, let stand, and remove air bubbles .

[0057] 2) Inject the chitosan solution in step (1) into the mould, let it stand at 4°C for 12h, transfer to -20°C for 24h, and put it into a freeze dryer for 48h.

[0058] 3) Soak the porous scaffold in step (2) in 0.5% sodium hydroxide solution to neutralize the residual acetic acid, then soak and wash it with deionized water until it is neutral; freeze-dry it again.

[0059] 4) Utilize the improved Hummers method to prepare graphite oxide:

[0060] ① Dissolve 1.5g of sodium nitrate in 69ml of concentrated sulfuric acid, add 2g of graphite,...

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Abstract

The invention provides a method for preparing a chitosan three-dimensional scaffold having graphene oxide coatings of different reducing degrees. The method specifically comprises the following steps: preparing a chitosan scaffold material having a porous structure by a freeze drying method; then soaking the chitosan scaffold material in graphene oxide dispersion fluids of different concentrations; adsorbing graphene oxide sheets to pore walls of chitosan through the electrostatic interaction and the hydrogen-bond interaction; drying, and finally carrying out gradient heating and reducing to obtain the chitosan scaffold which has greatly improved mechanical strength and has graphene oxide coatings of different reducing degrees. The chitosan scaffold has a good application prospect in the aspect of neural tissue engineering. The method provided by the invention is simple to operate and can be used for preparing a great amount of desired product.

Description

Technical field: [0001] The invention relates to a method for preparing graphene-coated chitosan scaffolds with different reduction degrees. The invention utilizes the self-assembly principle of graphene oxide sheets and the electrostatic and hydrogen bonds between oxygen-containing functional groups and chitosan scaffold materials Function, realized the coating of graphene oxide on the chitosan scaffold, and finally obtained the composite scaffold of reduced graphene oxide and chitosan with tissue engineering application prospect through thermal reduction method. Background technique: [0002] Life and regeneration engineering, including the ability of humans to manipulate the nervous system, biological cells, tissues and organs in the future, is considered to be one of the disciplines involved in the sixth technological revolution in science and industry. Among them, regenerative technology, as an important technology of the fourth industrial revolution, has become the goa...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/30A61L27/20A61L27/50
CPCA61L27/20A61L27/303A61L27/50A61L27/56C08L5/08
Inventor 王欣文少林王赞
Owner JILIN UNIV
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