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A kind of detection method of cyanide content in milk and milk powder

A detection method, cyanide technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cyanide measurement interference, cyanide detection method has not been reported, etc., to achieve good stratification, accurate and reliable measurement, high sensitivity and selective effects

Active Publication Date: 2018-05-18
INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large amount of protein, fat and other substances in milk and milk powder, from pretreatment to instrumental analysis, it has seriously interfered with the determination of cyanide. As a result, this principle has not been applied to the detection of cyanide in dairy products.
[0006] In summary, there are no reports on the detection methods of cyanide in milk and milk powder

Method used

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  • A kind of detection method of cyanide content in milk and milk powder
  • A kind of detection method of cyanide content in milk and milk powder
  • A kind of detection method of cyanide content in milk and milk powder

Examples

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Embodiment 1

[0063] Example 1: Determination of cyanide content in commercially available milk and milk powder.

[0064] (1) Sample collection and preparation:

[0065] Buy 10 different brands or different types of milk (including breakfast milk, high-calcium milk, pure milk, etc.) and 10 milk powders (whole milk powder, skimmed milk powder, infant milk powder, etc.) from the supermarket. Milk powder samples were diluted with deionized water at a ratio of 1:10 (m / v), mixed, and then processed and tested.

[0066] (2) Sample pretreatment:

[0067] Take 2.0mL of the above milk or milk powder dilution, add an appropriate amount of 0.1mol / L potassium hydroxide solution to adjust its pH to about 10, add 400μL 0.05mol / L octadecyltrimethylammonium bromide solution and 2.0mL Pentafluorobenzyl bromide-dichloromethane (0.4%, v / v) solution, vortexed on a vortex mixer for 2min, then derivatized in a water bath at 30°C for 30min, and then derivatized at 10000r / min at 4°C Centrifuge at a rotational s...

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Abstract

A gas chromatography-tandem quadrupole mass spectrometry detection method for the detection of cyanide content in milk and milk powder, the sample is under alkaline conditions, using octadecyltrimethylammonium bromide as a phase transfer catalyst, and pentafluorobenzyl Bromine (PFB‑Br) is the derivatization reagent, and the cyanide ion (CN‑) is derivatized into the volatile derivative product pentafluorophenylacetonitrile (PFB‑CN), and gas chromatography‑tandem quadrupole mass spectrometry (GC‑MS / MS) detection, standard curve method quantification, obtains the cyanide content in the sample. Using the method of the invention to detect the content of cyanide in milk and milk powder has the advantages of simple operation and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a method for detecting cyanide content in milk and milk powder. Background technique [0002] Cyanide is a class of common highly toxic substances. Common inorganic cyanides include sodium cyanide, potassium cyanide and hydrogen cyanide. Cyanide decomposes into cyanide ions (CN - ), inhibit the activity of various enzymes in tissue cells, so that cells cannot use oxygen, resulting in intracellular suffocation, leading to central respiratory failure and death. my country's national standard "GB 5749-2006 Hygienic Standards for Drinking Water" stipulates that the limit of cyanide is 0.05mg / L. Foreign countries have more detailed and strict limit requirements for cyanide in food. For example, Russia stipulates that the limit of cyanide in drinking water is 0.035mg / L. The food matrix puts forward the limit requirements: canned stone fruit is 5mg / kg, and ot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 崔宗岩曹彦忠钱云开王飞贾光群张少博石玉秋黄学者
Owner INSPECTION & QUARANTINE TECH CENT OF QINHUANGDAO ENTRY EXIT INSPECTION & QUARANTINE BUREAU