Hypoglycemic health food added with Anemarrhena extract
An Anemarrhena Anemarrhena extract and hypoglycemic technology, applied in the food field, can solve the problems of liver and kidney damage, large side effects and other problems in patients
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Embodiment 1
[0014] Example 1: Preparation of Anemarrhena Extract 1
[0015] (a) Mix 5 kg of dried Anemarrhena anemarrhena and Zaprona chinensis (accounting for 10% of the total weight), pulverize, extract with 85% ethanol solution under heat reflux (20 L × 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol Extract (3L×3 times), concentrate under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract is dissolved in water, filtered, and filtered with AB-8 The resin is enriched with active components. First, 8 column volumes are washed with 12% ethanol to remove large polar components, and then 10 column volumes are eluted with 80% ethanol. ...
Embodiment 2
[0016] Example 2: Preparation of Anemarrhena Extract 2
[0017] (a) Mix 5kg of dried Herba japonica (accounting for 5% of the total weight), pulverize, extract with 80% ethanol solution under heat reflux (20L×3 times), combine the extracts, concentrate until there is no alcohol smell to obtain ethanol extraction and concentration (b) Dilute the ethanol extraction concentrate obtained in step (a) with water (3 L), and extract with petroleum ether (3 L × 3 times), ethyl acetate (3 L × 3 times) and water-saturated n-butanol successively ( 3L×3 times), concentrated under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract was dissolved in water, filtered, enriched with AB-8 type macroporous resin To collect the active ingredients, wash 9 column volumes with 8% ethanol to remove large polar components, then elute 12 column volumes with 75% ethanol, collect the 75% ethanol eluate, and concentrate under r...
Embodiment 3
[0018] Example 3: Preparation of Anemarrhena Extract 3
[0019] (a) Mix 5kg of dried Herba japonica (accounting for 15% of the total weight), crush, extract with 90% ethanol solution under heat reflux, combine the extracts (20L×3 times), concentrate until there is no alcohol smell to obtain ethanol extraction and concentration (b) Dilute the ethanol extraction concentrate obtained in step (a) with water (3L), and then use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L × 3 times) extraction, concentrated under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract was dissolved in water, filtered, enriched with AB-8 type macroporous resin To collect the active ingredients, wash 7 column volumes with 15% ethanol to remove large polar components, then elute 8 column volumes with 85% ethanol, collect the 85% ethanol eluate, and concentrate under reduced pressure...
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