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A detection method for distinguishing types of sheepskin

A detection method and sheepskin technology, applied in the field of cultural relic detection, can solve the problems of appearance, performance difference, unusable leather, unsuitable for identification of cultural relics, etc., and achieve the effect of high sensitivity, strong specificity, and avoiding the interference of other proteins.

Active Publication Date: 2017-06-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above method can only be distinguished by the appearance and performance of the leather, but the appearance and performance of the cultural relics are quite different after unearthed, so it is not suitable for the identification of cultural relics, and can only distinguish different species of leather, and cannot be used for the same species of leather

Method used

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  • A detection method for distinguishing types of sheepskin
  • A detection method for distinguishing types of sheepskin

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Experimental program
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Embodiment 1

[0038] A detection method for distinguishing sheepskin types, comprising the following steps:

[0039]A) Dissolve 0.05g of sheepskin test sample in 500mL of EB elution buffer and stir evenly. After standing still, take 85μL of supernatant and add them to wells A, B, E and F of the microtiter plate. Add 85 μL of PBS buffer solution to the wells C and D of the ELISA plate, and put the wells A, B, C, D, E, and Wells in row F were used as experimental control 1, experimental control 2, blank control 1, blank control 2, negative control 1, and negative control 2; the microplate plate was refrigerated at 4°C.

[0040] B) Add 200 μL of blocking solution to the wells of each row of the ELISA plate, block at 37°C for 1.5 hours, suck out the liquid in the wells, and wash with PBS buffer 3 times, 3 minutes each time.

[0041] C) Add 85 μL of rabbit anti-goatskin type I collagen antibody diluted 6500 times with blocking solution to wells A and C of the microplate respectively; add 85 μL ...

Embodiment 2

[0048] A) Dissolve 0.01g of sheepskin test sample in 1000mL of EB elution buffer and stir evenly. After standing still, take 120μL of supernatant and add them to wells A, B, E and F of the ELISA plate. Add 120 μL of PBS buffer solution to the wells of row C and row D of the microtiter plate, and put the wells of row A, row B, row C, row D, and row E of the microtiter plate Wells in row F were used as experimental control 1, experimental control 2, blank control 1, blank control 2, negative control 1, and negative control 2; the microplate plate was refrigerated at 4°C.

[0049] B) Add 300 μL of blocking solution to the wells of each row of the ELISA plate, block at 36°C for 2 hours, suck out the liquid in the wells, and wash with PBS buffer 3 times, 3 minutes each time.

[0050] C) Add 120 μL of rabbit anti-goatskin type I collagen antibody diluted 1000 times with blocking solution to wells A and C of the microplate respectively; add 120 μL to wells B and D of the microplate r...

Embodiment 3

[0057] A) Dissolve 0.1g of sheepskin test sample in 100mL of EB elution buffer and stir evenly. After standing still, take 50μL of the supernatant and add it to the wells A, B, E and F of the microtiter plate. Add 50 μL of PBS buffer solution to the wells C and D of the microtiter plate respectively, and put the wells A, B, C, D, E, and Wells in row F were used as experimental control 1, experimental control 2, blank control 1, blank control 2, negative control 1, and negative control 2; the microplate plate was refrigerated at 4°C.

[0058] B) Add 100 μL of blocking solution to the wells of each row of the ELISA plate, block at 38°C for 1 hour, suck out the liquid in the wells, and wash with PBS buffer 3 times, 3 minutes each time.

[0059] C) Add 50 μL of rabbit anti-goatskin type I collagen antibody diluted 12,000 times with blocking solution to wells A and C of the microplate respectively; add 50 μL to wells B and D of the microplate respectively Dilute rabbit anti-sheep ...

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Abstract

The invention relates to the technical field of cultural relic detection, and discloses a detection method for distinguishing sheep skin types. The ingredients of type I collagen of different types of sheep skin are detected in indirect enzyme-linked immunosorbent assay through a goat skin and sheep skin type I collagen antibody prepared through different marker peptides, that is, an elisa plate is coated with sheep skin type I collagen eluent, then a rabbit-anti-I-type collagen antibody is added to be combined with an antigen to generate an antigen-antibody complex, after washing is performed, an alkaline phosphatase goat-anti-rabbit IgG (H+L) antibody is added to generate an antibody-antigen-antibody complex, washing is performed, a substrate color developing solution is added, a substrate is enzymatically catalyzed into a colored product, and qualitative analysis can be performed according to the product color changing degree. The method is simple, rapid and convenient to operate and high in sensitivity, interference of other protein can be avoided, and the specificity is high.

Description

technical field [0001] The invention relates to the technical field of cultural relic detection, in particular to a detection method for distinguishing sheepskin types. Background technique [0002] Sheepskin has been widely used in the north and northwest regions since ancient times, and it is mostly used as clothing and writing carriers. It is a precious wealth of our country. There are two main types of sheepskin, one is sheepskin, which is characterized by light and thin skin, soft, smooth and delicate feel, small pores, evenly distributed irregularly, and oblate shape, which is mostly used to make high-end clothing. Another kind of goatskin, the structure is stronger than sheepskin, so the tensile strength is better than sheepskin. Because the surface layer of the leather is thicker than sheepskin, it is more wear-resistant than sheepskin, rougher, and feels slightly worse. It is mostly used to make wear-resistant products such as hunting gear. [0003] In the face of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6881
Inventor 胡智文游秋实王秉刘意
Owner ZHEJIANG SCI-TECH UNIV
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