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A biomarker group for diagnosis of coronary atherosclerosis and stable angina

A technology for coronary atherosclerosis and stable angina pectoris, which is applied in the field of biochemistry to achieve the effects of improving diagnostic convenience, promoting standardization and high sensitivity

Active Publication Date: 2017-10-27
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] No one has used plasma metabolite levels to diagnose coronary heart disease

Method used

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  • A biomarker group for diagnosis of coronary atherosclerosis and stable angina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening characterization of differential metabolites in plasma between patients with coronary atherosclerosis and patients with stable angina

[0026] 1. Objects and methods

[0027] 1. The source of the specimen

[0028] After obtaining the consent of the patients, the peripheral venous blood plasma of 480 patients with coronary atherosclerosis, 280 patients with stable angina pectoris and 350 healthy people in Jiangsu Provincial People's Hospital from September 2010 to June 2015 were collected. Confirmed by coronary angiography. The age and sex of healthy people were matched with patients with coronary atherosclerosis and stable angina pectoris. All patients with coronary atherosclerosis, patients with stable angina pectoris and healthy people have normal heart, lung, liver, kidney and hematopoietic functions.

[0029] The time of blood collection was in the morning on an empty stomach.

[0030] 2. Main reagents

[0031] Acetonitrile and formic acid ...

Embodiment 2

[0052] Example 2: Construction of ROC curves to verify the ability of six differential metabolites to diagnose coronary atherosclerosis and stable angina pectoris

[0053] Receiver operating curve (ROC) method was used for verification, and the expression levels of differential metabolites in the plasma of patients with stable angina pectoris and patients with coronary atherosclerosis were used to diagnose and distinguish patients with stable angina pectoris and patients with coronary atherosclerosis Ability. The results showed that the six differential metabolites of glutathione, β-hydroxybutyric acid, proline, fumaric acid, phenylalanine, and lactic acid alone were used to diagnose patients with stable angina pectoris and patients with coronary atherosclerosis The ability is strong, and the area under the ROC curve (AUC) is greater than 0.7, which has clinical diagnostic significance; when combined for diagnosis, with the increase of the number of combinations, the AUC furth...

Embodiment 3

[0062] Embodiment 3: the preparation of detection kit

[0063] A detection kit has been prepared based on the metabolic markers provided by the present invention, and the kit includes the following components:

[0064] Standards of metabolic markers: including glutathione, β-hydroxybutyric acid, proline, fumaric acid, phenylalanine, lactic acid, each standard is packaged separately;

[0065] Plasma metabolite extraction solvent: 100% acetonitrile and 20% acetonitrile aqueous solution (for UPLC-Q / TOF-MS sample preparation); a mixed solution of methanol, chloroform and water in a ratio of 2.5:1:1, methoxyaminopyridine and N-Methyl-N-trimethylsilyl trifluoroacetamide (for GC-Q / MS sample preparation); 20% acetonitrile aqueous solution can be used as dissolved standard in UPLC-Q / TOF-MS screening and characterization Solvent; in GC-Q / MS screening and characterization, use the plasma metabolite extraction solvent to prepare the standard solution according to the sample preparation m...

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Abstract

A marker group for making diagnosis to distinguish coronary arterial atherosclerosis from stable angina pectoris. The marker group comprises one or more of glutathione, β-hydroxybutyric acid, proline, fumaric acid, phenylalanine and lactic acid. When a single marker is used for making diagnosis to distinguish patients with the stable angina pectoris from patients with the coronary arterial atherosclerosis, the areas under curve (AUC) of a ROC curve are all 0.7 or larger, and the marker has a clinical diagnosis significance; when the marker is used for diagnosis in combination, with the increase of the combination number, the AUC is further increased and reaches 0.988 when 6 markers are combined, and the sensitivity and the specificity are 98.5% and 98.3% respectively under the optimum cutoff value. The metabolic marker group can be used to accurately making diagnosis to distinguish the coronary arterial atherosclerosis from the stable angina pectoris, and has high sensitivity and specificity.

Description

technical field [0001] The invention belongs to the field of biochemistry and relates to metabolic markers for diagnosis and classification of coronary heart disease, in particular to a group of metabolic markers for diagnosing and distinguishing coronary atherosclerosis and stable angina pectoris. Background technique [0002] Coronary heart disease, also known as ischemic heart disease, involves atherosclerosis in the arteries supplying myocardial blood, that is, coronary atherosclerotic lesions cause vascular lumen narrowing or plaque formation or even rupture and complete blockage, resulting in myocardial ischemia and hypoxia. Or necrosis leads to cardiac ischemic disease, which leads to a series of serious cardiovascular events such as clinical angina pectoris and myocardial infarction. Coronary heart disease is the main killer of human health. It has the characteristics of high incidence rate, high disability rate, high recurrence rate, high mortality rate, and many co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2800/323G01N2800/324
Inventor 齐炼文李萍朱伟陈彦范勇
Owner CHINA PHARM UNIV
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