Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method

A medium and trace element and inductive coupling technology, which is applied in the direction of measuring devices, preparation of test samples, material analysis by electromagnetic means, etc., can solve the problems of pollution, long time consumption, large amount of reagents, etc., and achieve simple pretreatment, sample The effect of less dosage and lower cost

Inactive Publication Date: 2016-07-20
GUANGZHOU SHENGXIN BIOTECH CO LTD
View PDF0 Cites 42 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhang Sujing’s paper titled “Detection Method of 34 Elements in Blood and Urine and Its Application Research” disclosed a method for the detection of 34 elements in blood by ICP-MS. A large amount is used, which is easy to cause pollution and loss of volatile components, and the amount of sample to be processed at one time is small and takes a long time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method
  • Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method
  • Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The detection of trace element magnesium, manganese, calcium, iron, copper, zinc and lead in embodiment one human body whole blood

[0030] 1. Instrument: Inductively Coupled Plasma Mass Spectrometer

[0031] 2. Solution preparation:

[0032] Preparation of diluent: add deionized water at a volume ratio of 1:999 to prepare a mixed solution of nitric acid and triton with a mass concentration of 0.1%, which is the diluent.

[0033] Preparation of tuning solution: prepare the tuning stock solution with nitric acid solution with a volume concentration of 0.2% to prepare a tuning solution with a mass concentration of 10 μg / L for use. The tuning elements are lithium, cobalt, yttrium, thallium, cerium, indium and barium.

[0034] Preparation of internal standard working solution: prepare the internal standard stock solution with nitric acid solution with a volume concentration of 0.2% to prepare an internal standard working solution with a mass concentration of 100 μg / L for u...

Embodiment 2

[0047] The mensuration of embodiment 2 detection method detection limit

[0048] Take 0.25mL deionized water, mix deionized water and diluent at a ratio of 1:30 as the sample blank, repeat the sample blank 20 times for intra-batch determination, calculate the mean value X and standard deviation SD, and use 3 times the standard deviation SD as the detection limit. The detection limits of each element are shown in Table 2.

[0049] Table 2 The detection limit of each element detection method

[0050] Analyte Magnesium (mg / L) Manganese (μg / L) Iron (mg / L) Copper (μg / L) Zinc (mg / L) Lead (μg / L) Calcium (mg / L) average 0.023 0.383 0.998 1.400 0.008 0.632 0.233 standard deviation 0.009 0.113 0.830 0.636 0.002 0.064 0.040 The detection limit 0.026 0.339 2.491 1.908 0.005 0.192 0.120

Embodiment 3

[0051] Embodiment 3 detection method precision measurement

[0052] 1. Preparation of quality control products:

[0053] The quality control product is Seronom TM TraceElementsWholeBloodL-2 (purchased from Auspan), accurately draw 3mL of ultrapure water into the bottle with a pipette according to the instructions, let it stand for 30min, then invert the vial, after standing for 1 hour, shake it gently and divide it, store in Store in a -20°C refrigerator and use within the stable period.

[0054] The second quality control product is whole blood of self-breeding bovine.

[0055] Add the standard solution of each element to be tested in the quality control product as the sample to be tested, so that the concentration of each element in the quality control product is reflected in the medically determined level range of the whole blood. Among them, lead is 100.0 μg / L, manganese is 6.6 μg / L L, copper 720.0μg / L, zinc 3.7mg / L, iron 557.2mg / L, magnesium 50.4mg / L, calcium 84.0mg / L....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for detecting trace elements in human body whole blood through an inductively coupled plasma mass spectrometry method and belongs to the technical field of biotechnical detection. The method comprises the following steps: (1) pre-treating a whole blood sample; (2) detecting a mixed element standard solution and a to-be-detected sample solution by an inductively coupled plasma mass spectrometer; and (3) performing quantitative analysis on a detection result and calculating the content of to-be-detected elements in the human body whole blood sample. The method provided by the invention can be used for simultaneously detecting the content of trace elements, such as magnesium, manganese, calcium, iron, copper, zinc and lead, in the human body whole blood; the sample dosage is less; the pretreatment is simple; the cost is low; the whole detection process is short in time and high in efficiency; the detection sensitivity and precision andthe detection stability are high.

Description

technical field [0001] The invention belongs to the technical field of biological detection and relates to a method for measuring trace elements in human whole blood by inductively coupled plasma mass spectrometry. Background technique [0002] The change of trace elements in human body, especially the content of trace elements in blood is directly related to the physiological and pathological conditions of human body. Whole blood trace elements magnesium, manganese, calcium, iron, copper, and zinc are important components of large biomolecules such as enzymes, hormones, and vitamins. They play important physiological and biochemical functions in the human body, and they can affect the human immune system. There is a causal relationship between the occurrence of diseases, such as calcium deficiency can cause enchondroma, osteoporosis and rickets, while excessive calcium intake will inhibit the absorption of zinc and easily cause diseases such as gallstones; iron is one of th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/38
CPCG01N1/38G01N27/64
Inventor 程雅婷文国学陈静宜陈文彬李超
Owner GUANGZHOU SHENGXIN BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap