Method for detecting trace elements in human body whole blood through inductively coupled plasma mass spectrometry method

A medium and trace element and inductive coupling technology, which is applied in the direction of measuring devices, preparation of test samples, material analysis by electromagnetic means, etc., can solve the problems of pollution, long time consumption, large amount of reagents, etc., and achieve simple pretreatment, sample The effect of less dosage and lower cost

CN105784831AInactive Publication Date: 2016-07-20GUANGZHOU SHENGXIN BIOTECH CO LTD
0 Cites 42 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2016-07-20
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a method for detecting trace elements in human body whole blood through an inductively coupled plasma mass spectrometry method and belongs to the technical field of biotechnical detection. The method comprises the following steps: (1) pre-treating a whole blood sample; (2) detecting a mixed element standard solution and a to-be-detected sample solution by an inductively coupled plasma mass spectrometer; and (3) performing quantitative analysis on a detection result and calculating the content of to-be-detected elements in the human body whole blood sample. The method provided by the invention can be used for simultaneously detecting the content of trace elements, such as magnesium, manganese, calcium, iron, copper, zinc and lead, in the human body whole blood; the sample dosage is less; the pretreatment is simple; the cost is low; the whole detection process is short in time and high in efficiency; the detection sensitivity and precision andthe detection stability are high.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biological detection and relates to a method for measuring trace elements in human whole blood by inductively coupled plasma mass spectrometry. Background technique

[0002] The change of trace elements in human body, especially the content of trace elements in blood is directly related to the physiological and pathological conditions of human body. Whole blood trace elements magnesium, manganese, calcium, iron, copper, and zinc are important components of large biomolecules such as enzymes, hormones, and vitamins. They play important physiological and biochemical functions in the human body, and they can affect the human immune system. There is a causal relationship between the occurrence of diseases, such as calcium deficiency can cause enchondroma, osteoporosis and rickets, while excessive calcium intake will inhibit the absorption of zinc and easily cause diseases such as gallstones; iron is one of th...

Examples

Embodiment 1

[0029] The detection of trace element magnesium, manganese, calcium, iron, copper, zinc and lead in embodiment one human body whole blood

[0030] 1. Instrument: Inductively Coupled Plasma Mass Spectrometer

[0031] 2. Solution preparation:

[0032] Preparation of diluent: add deionized water at a volume ratio of 1:999 to prepare a mixed solution of nitric acid and triton with a mass concentration of 0.1%, which is the diluent.

[0033] Preparation of tuning solution: prepare the tuning stock solution with nitric acid solution with a volume concentration of 0.2% to prepare a tuning solution with a mass concentration of 10 μg / L for use. The tuning elements are lithium, cobalt, yttrium, thallium, cerium, indium and barium.

[0034] Preparation of internal standard working solution: prepare the internal standard stock solution with nitric acid solution with a volume concentration of 0.2% to prepare an internal standard working solution with a mass concentration of 100 μg / L for u...

Embodiment 2

[0047] The mensuration of embodiment 2 detection method detection limit

[0048] Take 0.25mL deionized water, mix deionized water and diluent at a ratio of 1:30 as the sample blank, repeat the sample blank 20 times for intra-batch determination, calculate the mean value X and standard deviation SD, and use 3 times the standard deviation SD as the detection limit. The detection limits of each element are shown in Table 2.

[0049] Table 2 The detection limit of each element detection method

[0050] Analyte Magnesium (mg / L) Manganese (μg / L) Iron (mg / L) Copper (μg / L) Zinc (mg / L) Lead (μg / L) Calcium (mg / L) average 0.023 0.383 0.998 1.400 0.008 0.632 0.233 standard deviation 0.009 0.113 0.830 0.636 0.002 0.064 0.040 The detection limit 0.026 0.339 2.491 1.908 0.005 0.192 0.120

Embodiment 3

[0051] Embodiment 3 detection method precision measurement

[0052] 1. Preparation of quality control products:

[0053] The quality control product is Seronom TM TraceElementsWholeBloodL-2 (purchased from Auspan), accurately draw 3mL of ultrapure water into the bottle with a pipette according to the instructions, let it stand for 30min, then invert the vial, after standing for 1 hour, shake it gently and divide it, store in Store in a -20°C refrigerator and use within the stable period.

[0054] The second quality control product is whole blood of self-breeding bovine.

[0055] Add the standard solution of each element to be tested in the quality control product as the sample to be tested, so that the concentration of each element in the quality control product is reflected in the medically determined level range of the whole blood. Among them, lead is 100.0 μg / L, manganese is 6.6 μg / L L, copper 720.0μg / L, zinc 3.7mg / L, iron 557.2mg / L, magnesium 50.4mg / L, calcium 84.0mg / L....