A method of using ultraviolet light to prevent early blight of tomato in greenhouses
A tomato early blight and ultraviolet lamp technology is applied in the field of using ultraviolet lamps to prevent and control tomato early blight in greenhouses. Effect
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example 1
[0015] Example 1, according to the above-mentioned content of the invention, propose detailed operation steps
[0016] 1. Determination of the pathogenicity of Phytophthora tomato by ultraviolet light irradiation
[0017] Collect the diseased strains of Phytophthora tomato, isolate and purify the pathogens in the laboratory, and obtain purified pathogenic bacteria; pick the purified pathogenic mycelia and inoculate them on potato agar medium, cultivate them at 25°C for 48 hours, and then open the culture dish, and placed under different UV irradiation conditions;
[0018] ① Pick colonies from the UV-treated petri dish at the center of the petri dish, inoculate them on PDA medium, and culture them for 48 hours under dark conditions at 25°C to measure the growth of the colonies;
[0019] serial number 1 2 3 4 5 6 7 Irradiation time (min) 15 30 45 60 75 90 105 Average spore production (pcs / ml) 10 8
10 6
10 6
10 5
10 4
10 4
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example 2
[0030] Example 2, the control method of the present invention is compared with conventional pesticide control, and measures the control effect;
[0031] (1) At the early stage of disease occurrence, 75% chlorothalonil wettable powder 600 times liquid, 50% thiophanate wettable powder 600 times liquid, 70% mancozeb 700 times liquid were used alternately, and sprayed every 10 days Medicine 1 time, continuous spray 3 times.
[0032] (2) Set up a 100w UV-B lamp tube (240nm-340nm) in the greenhouse; use a 0.08mm cellulose acetate film to coat the UV-C ultraviolet lamp with a wavelength component below 253mm; about 1.5 meters from the ground, mutually Install ultraviolet lamps vertically, one for every 20 to 30 cubic meters of space, and the ultraviolet rays are irradiated at an angle of 60 to 80 degrees to the horizontal plane; When it is stable, start to irradiate for 45 minutes, which has an inhibitory effect on tomato early blight. Continuously irradiate for 7 days, and then irr...
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