Infectious bursal disease virus a11 strain and its application

A bursal disease and infectious technology, applied in the field of microorganisms, can solve problems such as difficulty in eradication, and achieve the effect of fast speed and strong immune protection effect.

Active Publication Date: 2019-07-05
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the biological characteristics of IBDV such as strong resistance, mutability, and the ability to carry and spread in a variety of birds, it is difficult to eradicate the disease

Method used

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  • Infectious bursal disease virus a11 strain and its application
  • Infectious bursal disease virus a11 strain and its application
  • Infectious bursal disease virus a11 strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] (1) Isolation of epidemic strains of infectious bursal disease virus

[0026] 1 material

[0027] Infectious bursal disease materials J3 (2013), A4 (2013), S8 (2013), S9 (2014), A11 (2014), S14 (2015) and J17 (2015) are from the past three years (2013~ 2015) In chicken farms (IBD vaccine immunized flocks) in Jiangsu (J), Anhui (A) and Shandong (S), sick chickens with clinical IBD had swelling, hemorrhage, and inflammatory exudates in the bursa of Fabricius and other diseases. SPF eggs (purchased from Shandong Yufeng Agricultural Science and Technology Co., Ltd. Specific Pathogen Free Breeding Farm) were hatched into 9-day-old chicken embryos in the laboratory, and raised to 6-week-old chicks in a clean animal room. The DF-EGFP-miR-9 cell line is a chicken embryo fibroblast cell line that highly expresses gga-miR-9 and eGFP genes (Ouyang Wei, et al. Chicken embryo fibroblast cell line that highly expresses gga-miR-9* .China, National Invention Patent No. ZL20131056524...

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Abstract

The invention discloses an infectious bursal disease virus strain A11 and application thereof, and belongs to the field of the microbial technology. Infectious bursal disease viruses are separated from infectious bursal disease vaccine immunity failure chicken flocks appearing in recent years, biological characteristic analysis is carried out on obtained virus isolate strains, and the infectious bursal disease virus strain A11 with good biological characteristics is screened out from the obtained virus isolate strains and is a currently-popular infectious bursal disease virus with super-strong virulence. The virulence of the virus is attenuated with a chicken embryo and cell continuous passage method to culture the low-virulence strain A11, the low-virulence strain A11 has super-strong reproductivity in cells, the virus yield in cells reaches up to 10<11> TCID50 / 0.1 ml, immune protection resisting infectious bursal disease viruses can be rapidly generated by immunizing chicks with the strain A11, immune protection can be achieved after 1 week, and the strain A11 has huge application value in developing new specific infectious bursal disease vaccines.

Description

technical field [0001] The invention relates to an infectious bursal disease virus A11 strain, which has excellent biological characteristics, high yield in cells, strong and fast immunity of chicks, and belongs to the technical field of microorganisms. Background technique [0002] Infectious Bursal Disease (IBD) is caused by Infectious Bursal Disease Virus (IBDV) and is characterized by invading chick lymphoid tissue, especially the central immune organ - the bursa of infectious diseases. The disease not only causes the death of sick animals, but also leads to immunosuppression of the body, which reduces the immune defense ability of the body and the failure of vaccine immunization, which occurs in all chicken raising areas in the world. [0003] Due to the biological characteristics of IBDV, such as strong resistance, easy mutation, and the ability to be carried by a variety of birds, it is difficult to eradicate the disease. In recent years, due to the widespread use o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
CPCA61K39/12A61K2039/5254A61K2039/552C12N7/00C12N2720/10021C12N2720/10034
Inventor 王永山梅永杰欧阳伟诸玉梅潘群兴王晓丽孟凯夏兴霞毕振威王晶宇
Owner JIANGSU ACAD OF AGRI SCI
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