Primer, probe and kit for detecting canine parvovirus and detection method

Abstract

The invention relates to a primer, probe and kit for detecting canine parvovirus and a detection method.According to the fluorogenic quantitative PCR primer for detecting canine parvovirus, an upstream detection primer CPV-F has the nucleotide sequence as shown in the SEQ ID NO.1, and a downstream detection primer CPV-R has the nucleotide sequence as shown in the SEQ ID NO.2.The probe has the nucleotide sequence as shown in the SEQ ID NO.3.The kit comprises the primer and the probe.The fluorogenic quantitative PCR primer, probe and kit for detecting canine parvovirus have the advantages that operation is easy, cost is low, sensitivity is high and specificity is high, and can guarantee clinical detection.

Description

technical field [0001] The invention belongs to the field of virus detection, in particular to a primer, a probe, a kit and a detection method for fluorescent quantitative PCR for directly detecting canine parvovirus in one step. Background technique [0002] Canine parvovirus (Canine Parvovirus, CPV) is a severe infectious virus that causes canine animals with severe vomiting, hemorrhagic enteritis, non-suppurative myocarditis and significant reduction of white blood cells. Canine parvovirus is a highly contagious, highly contagious disease characterized by vomiting, diarrhea, and leukopenia. Cases of natural infection with canine parvovirus occur frequently, even after CPV vaccine immunization, and the mortality rate of puppies is very high (20%-30%). With the rapid development of the dog breeding industry, especially the substantial increase in the number of experimental dogs and pet dogs, the parvovirus infection of dogs is becoming more and more serious, which has caus...

AI Technical Summary

Problems solved by technology

[0004] At present, the method of detecting canine parvovirus is mainly to observe clinical symptoms. There are test strips for virus detection methods, and there are also reports that traditional PCR and fluorescent quantitative PCR are used, but the accuracy of test strips is generally poor, and the traditional PCR method takes a long time. and low sensitivity

[0005] However, although the current fluorescent quantitative PCR has improved sensitivity and accuracy compared with the traditional PCR method and test strip detection method, it still has defects such as high detection cost, long time-consuming, and complicated operation.

For example, in fluorescent quantitative PCR, it is necessary to use nucleic acid extraction solution to pretreat samples and extract viral nucleic acids (such as: DNA and RNA, etc.). If the quality of nucleic acid extraction is not good, it will affect subsequent experiments, especially RNA. Prone to degradation, so the extraction steps are more complicated and strict

[0006] There is also a method for detecting canine parvovirus using biological barcodes, but this method needs to be used in conjunction with the biological barcodes used to detect canine parvoviruses, the complementary probe NP chains used in conjunction with the barcodes, and their nucleotide sequences, and the method is more efficient. Complicated and costly, it is not conducive to large sample size detection and epidemiological investigation research

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