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Rhodococcus ruber XS-2 strain and biological microbial inoculant, and preparation method and application thereof

A technology of Rhodococcus erythrococcus and XS-2, applied in the field of agricultural biology, can solve the problems of reducing pesticides and affecting the final use of pesticides, so as to reduce pollution, promote healthy and sustainable agricultural development, and improve soil structure and crop quality.

Active Publication Date: 2016-08-03
BIOLOGY INST OF HEBEI ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of repair method will affect the final use effect of pesticides due to the excessive reduction of pesticide content.

Method used

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  • Rhodococcus ruber XS-2 strain and biological microbial inoculant, and preparation method and application thereof
  • Rhodococcus ruber XS-2 strain and biological microbial inoculant, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Screening, separation and degradation characteristics of carbendazim in embodiment 1 bacterial strain XS-2

[0028] (1) Take soil samples: collect soil samples in vegetable planting areas where carbendazim and other pesticides are often sprayed, shovel off 2-3cm of topsoil, and take 10g of soil; put them into sterilized kraft paper bags, and seal the bags and record the sampling location, environment and date.

[0029] (2) Strain enrichment: Take the soil and put it into a conical flask containing a low-concentration carbendazim enrichment medium, and culture it on a shaking table at 30°C for 5 days. Passage 10% of the inoculum into high-concentration enrichment medium to continue shaking culture, and successively subculture enrichment culture (carbendazim concentration from 300-800mg / L).

[0030] (3) Isolation and screening: Take 0.1-0.2mL of enrichment solution and spread it evenly on the medium plate containing carbendazim for isolation and purification, culture at ...

Embodiment 2

[0041] Embodiment 2 Bacterial strain XS-2 strain identification

[0042] According to the experimental content and test methods recorded in the "Common Bacteria System Identification Manual", the screened strain XS-2 was identified for its morphology, physiology and biochemistry. After the strain was cultured on PB plate medium for 24 hours, the colony characteristics were: the colony was round, opaque, dry, with raised surface, neat edges, and orange-red color; the cell morphology characteristics were: the cells in the early growth stage were rod-shaped, and the cells The length difference is large, showing polymorphism, and becomes ellipsoid after the culture time increases. Bacteria XS-2I is an aerobic bacteria, Gram staining is positive, does not produce spores, physiological and biochemical characteristics are V.P reaction positive, methyl red reaction negative, catalase positive, oxidase positive, can not liquefy gelatin, can use cyclopaste Ethin, citrate, ribose, a-D-g...

Embodiment 6

[0057] The preparation of embodiment 6 soil remediation bacterial agent solid preparation

[0058] The preserved strains of Rhodococcus erythrococcus XS-2, Serratia A5, Bacillus brevis BBC-3 and Bacillus subtilis BSD-2 were streaked and transferred to PB medium plates, and cultured at 30°C for 2 days. The bacterial lawn was inoculated in the PB seed medium, and the shaker cultured at 30°C for 24-36 hours to obtain the seed solution.

[0059] The seed solution is inoculated into a large amount of fermentation medium at an inoculation amount of 10% by volume, and cultured at 30° C. for 36-48 hours to obtain a fermentation solution. Each fermented liquid and substrate are mixed evenly according to the ratio shown in Table 4, and further obtained solid powder by spray drying, and then mixed according to the ratio of each strain shown in Example 3, the cell concentration ≥ 1.0 × 10 10 cfu / g to obtain a solid composite soil remediation bacterial agent.

[0060] The mass ratio of e...

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Abstract

The invention belongs to the technical field of agricultural biology, and particularly discloses a Rhodococcus ruber XS-2 strain of which the collection number is CGMCC No.11562. The Rhodococcus ruber XS-2 strain has the function of degrading chemical pesticides. The invention also discloses a microbial preparation containing the Rhodococcus ruber and a preparation method thereof. The microbial preparation is compounded from the Rhodococcus ruber, Serratieae Bacillus subtilis and Brevibacillus brevis. The microbial preparation has the functions of restoring pesticide-polluted soil, preventing diseases and promoting growth, has high environmental safety, and has favorable development and application prospects.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a rhodococcus rubella XS-2, its biological agent, a preparation method and an application. Background technique [0002] At present, the total application amount of organic pesticides in my country has reached 1.312 million tons (patented drugs), and the average application amount is 1.40g / m 2 , has far surpassed that of developed countries. With the improvement of people's living standards and improvement of diet structure, the planting area of ​​vegetables and melons and fruits has increased significantly, and the amount of pesticides used is 1-2 times higher than that of food crops. About 80% of the pesticides used in production enter the soil, except some of them are absorbed by plants or escape into the atmosphere, resulting in serious pesticide residues in the soil. Excessive chemical pesticide pollution in the soil not only destroys the biodiversity ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/425C12R1/125
CPCB09C1/10C12N1/20C12N1/205C12R2001/01
Inventor 程辉彩习彦花张丽萍崔冠慧王宏伟邢清朝何强张根伟尹淑丽刘洪伟
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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