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Probiotics and microelement dietary supplement with function of alleviating lead toxicity

A dietary supplement and trace element technology, applied to the functions of food ingredients, biochemical equipment and methods, bacteria used in food preparation, etc., can solve problems such as swelling, nausea, etc. High, long shelf life effect

Active Publication Date: 2016-08-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dimercaptosuccinic acid (DMSA), a competitive antidote of DMSA, may cause certain side effects such as upper and middle abdominal pain and nausea after being absorbed into the human body

Method used

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  • Probiotics and microelement dietary supplement with function of alleviating lead toxicity
  • Probiotics and microelement dietary supplement with function of alleviating lead toxicity
  • Probiotics and microelement dietary supplement with function of alleviating lead toxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Effect of different freeze-drying protective agents on the survival rate of Lactobacillus plantarum CGMCC 5494

[0039] After activating Lactobacillus plantarum CGMCC 5494 in MRS liquid medium for two generations, it was inserted into 1L MRS liquid medium according to the inoculum size of 2%, and placed at 37°C for constant temperature expansion for 18 hours. Centrifuge at 5000 g for 15 min at 4°C, discard the supernatant to collect the cells, wash the cells twice with sterile phosphate buffered saline (PBS, 60 mmol / L, pH 6.5), and collect the cells. The above experiment was repeated 5 times, and 5 parts of bacteria were collected, and 25mL of sterile PBS solution, 150g / L sucrose solution, 100g / L trehalose solution, 50g / L sorbitol solution, and 100g / L skimmed milk powder solution were added as freeze-dried The protective agent was mixed and resuspended. Dispense 1 mL of the bacterial suspension containing the protective agent into sterile vials. Pre-free...

Embodiment 2

[0045] Example 2: Effect of trehalose concentration on freeze-dried activity of Lactobacillus plantarum CGMCC 5494 cells

[0046] Protectant solutions with different concentration gradients of trehalose concentrations of 100g / L, 150g / L, 200g / L, and 250g / L were prepared, and the expanded culture, centrifugation, washing and freezing of Lactobacillus plantarum CGMCC 5494 were carried out according to the process of Example 1 Drying, counting of living bacteria to investigate the effect of the concentration of trehalose on the freeze-drying activity of CGMCC 5494 cells. The results are shown in Table 3:

[0047] Table 3 The protective effect of trehalose with different concentration gradients on CGMCC 5494 during freeze-drying

[0048]

[0049] Note: Different letters represent significant differences between groups (P<0.05)

[0050] It can be seen from Table 3 that with the increase of trehalose concentration, the protective effect was significantly improved. However, expe...

Embodiment 3

[0051] Embodiment 3: the influence of skimmed milk concentration on the lyophilization activity of Lactobacillus plantarum CGMCC 5494 thallus

[0052] Preparation of skim milk concentration is 100g / L, 150g / L, 200g / L, 250g / L freeze-drying protective agent solution of different concentration gradients, according to the process of embodiment 1, carry out the expansion culture, centrifugation and washing of Lactobacillus plantarum CGMCC 5494 Compared with freeze-drying and viable count, the effect of the concentration of skim milk on the freeze-drying activity of Lactobacillus plantarum CGMCC 5494 was investigated. The results are shown in Table 4:

[0053] The impact of different concentrations of skimmed milk powder in table 4 on the survival rate of Lactobacillus plantarum CGMCC 5494

[0054]

[0055] Note: Different letters represent significant differences between groups (P<0.05)

[0056] It can be seen from Table 4 that with the increase of the concentration of skim mil...

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Abstract

The invention discloses a probiotics and microelement dietary supplement with the function of alleviating lead toxicity and belongs to the technical field of food. According to the invention, a strain undergoes freeze-drying to obtain strain powder which is used as a main drug, supplemented by an antioxidant substance and microelements, proper auxiliary materials are added, and the raw materials are treated by a direct powder compression method so as to prepare a tablet form of the probiotics and microelement dietary supplement. Active bacteria content of the probiotics and microelement dietary supplement is greater than active bacteria content of 109CFU / g of lactobacillus plantarum CGMCC 5494. The dietary supplement obtained by the method can reduce content of lead in mice exposed to lead, recover oxidative stress injury caused by lead exposure, raise activity of aminolevulinic acid dehydratase in mice exposed to lead, lower content of zinc protoporphyrin in mice exposed to lead, and recover mice exposed to lead from intellectual damage. The dietary supplement of the invention has a wide application prospect.

Description

technical field [0001] The invention relates to a dietary supplement of probiotics and trace elements capable of alleviating lead toxicity, belonging to the technical field of food. Background technique [0002] Lead is a silvery-white heavy metal with a bluish tint, which is easily oxidized and darkens in the air. Lead is widely used, it can effectively block X-rays and radioactive rays, and it is also the main material for making batteries. In addition, lead compounds are raw materials for many pigments. Lead pollution refers to the pollution caused by lead or lead compounds to the surrounding environment, and its concentration and chemical form are directly related to its degree of harm. The strong enrichment of lead makes it difficult to degrade in the environment and cannot be reduced through the purification of the environment itself. If it exceeds the normal range, it will not only endanger human health, but also lead to deterioration of environmental quality. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L33/15A23L33/16A23P10/28C12N1/20C12R1/25
CPCC12N1/20A23V2002/00A23V2400/169A23V2200/30A23V2250/1578A23V2250/1628A23V2250/51084A23V2250/51086A23V2250/708
Inventor 陈卫翟齐啸田丰伟刘洋赵建新王刚张秋香刘小鸣张灏
Owner JIANGNAN UNIV
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