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Dynamic cell printing micromixer

A cell printing and micro-mixer technology, which is applied to animal cells, vertebrate cells, mixers, etc., can solve the problems of physical damage of the mixed liquid, inability to be used as cells, and print a micro-mixer, so as to reduce the complexity of mechanism design, The mixing effect is obvious and the volume of the mixer cavity is small.

Active Publication Date: 2016-08-17
ZHEJIANG UNIV
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Problems solved by technology

[0007] Among the existing inventions and devices of some dynamic micro-mixers, there are many micro-mixers including ultrasonic mixing, electrokinetic mixing, centrifugal mixing and alternating pulse flow mixing. Although the mixer chamber is small in volume, it can mix in real time. However, due to the complex control method, low integration and serious physical damage to the mixed liquid, it cannot be used as a micro-mixer for cell printing.

Method used

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  • Dynamic cell printing micromixer
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specific example

[0062] The dynamic cell printing micro-mixer of the present invention was used to study the effects of the concentration of various growth factors and their gradient distribution on the growth of mouse epidermal fibroblasts.

[0063] Step 1. Prepare all the components. Disinfect the motor fixing block, upper and lower chambers, stirrer, rotating shaft, adjustable speed motor installation surface and its main shaft with 75% alcohol. Luer connector, quick connector, syringe, feed Tubes and other equipment are sterilized at high temperature, and finally all equipment that comes into contact with cells is sterilized by ultraviolet light.

[0064] Step 2: Assemble the components on the clean bench, and connect the feed hoses and quick connectors of the two input channels. Pass clean water at a constant temperature of 37 degrees from the quick connector, and preheat for 5 to 10 minutes. Start the stirring bar and keep the rotation speed of 7r / s, use 37°C PBS to pass through the fee...

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Abstract

The invention discloses a dynamic cell printing micromixer which comprises a mixer cavity with a discharge outlet in the bottom. The side wall of the mixer cavity is provided with at least two feed inlets. A stirrer is arranged in the mixer cavity. An adjustable-speed motor in linkage with the stirrer is installed outside the mixer cavity. Multiple guide grooves are formed in the periphery of the stirrer and attached to the inner side wall of the mixer cavity. The dynamic cell printing micromixer has the advantages of being good in biological compatibility, small in design size, easy to disassemble, assemble, sterilize and clean, convenient to integrate, high in mixing and response gradient, adjustable in stirring speed, small in damage to cell and the like, and has the wide prospect in the fields of medicine screening, gradient manufacturing, biological printing and the like.

Description

technical field [0001] The invention relates to the field of biomanufacturing engineering and the field of cell printing technology, in particular to a dynamic cell printing micro-mixer. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, the field of life sciences is ushering in a period of rapid development. Among them, with the emergence of 3D printing technology, the direction of traditional biological research is gradually turning to the field of biomanufacturing. It is expected to realize manufacturing in the fields of in vitro reconstruction and organ repair to repair or replace human diseased tissues and organs, greatly prolonging human life. [0003] In terms of industrial layout, industries that exceed about 40% of GDP are the space for the development of bio-manufacturing in the future. According to the research and analysis results conducted by OECD on 6 developed countries, the application of ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F7/16B01F15/00B01F15/02B01F15/06C12N5/071
CPCC12N5/0625C12N2501/10C12N2513/00B01F27/053B01F27/0724B01F27/092B01F27/116B01F27/84B01F2035/351B01F35/221422B01F35/3204B01F35/7174B01F35/92B01F2101/44
Inventor 贺永赵海明傅建中
Owner ZHEJIANG UNIV
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