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A circRNA marker, kit and gene chip for diagnosis of depression

A gene chip, depression technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, organic compound library, etc., can solve the problems of lack of specific biomarkers, increase of diagnostic misdiagnosis rate, lack of objective indicators in clinical diagnosis, etc. To achieve the effect of simplifying the diagnosis process and improving the diagnosis accuracy

Active Publication Date: 2021-05-18
张理义
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Problems solved by technology

[0006] More evidence shows that cirRNA plays a very important role in the fine-tuning of miRNA level, and regulates gene expression by competing with miRNA. Existing studies have confirmed that miRNA plays an important regulatory role in the pathogenesis of depression Therefore, cirRNA may be further involved in the pathological regulation of depression; and the lack of specific biomarkers results in the lack of objective indicators for clinical diagnosis and increases the rate of misdiagnosis. Therefore, it is necessary to explore specific biomarkers for the diagnosis and efficacy observation of depression cirRNA is of great significance and value for the diagnosis, treatment and prevention of depression

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  • A circRNA marker, kit and gene chip for diagnosis of depression
  • A circRNA marker, kit and gene chip for diagnosis of depression
  • A circRNA marker, kit and gene chip for diagnosis of depression

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[0039] The case group (MDD) consisted of patients from April 2014 to January 2015 in the 102 Hospital of the People's Liberation Army and Changzhou Maternal and Child Health Hospital. A total of 100 cases were enrolled. The enrolled patients all met the diagnostic criteria for MDD in the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV-TR). The diagnosis was made by 2 psychiatrists according to the diagnostic criteria in the Chinese version of the modified Structured Clinical Interview for DSM-IV, patient version (SCID-I / P) (First et al., 1995). The inter-psychiatrist reliability was 0.87, and finally the severity of anxiety disorders was assessed using 24 items in the HAMD scale. Inclusion criteria: 1. No use of antidepressant drugs within three months before enrollment; 2. Han nationality, aged 18 to 60 years; 3. No history of serious organic diseases (such as heart disease, diabetes, Parkinson's syndrome) ), there was no serious traumatic...

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Abstract

The invention belongs to the field of biotechnology, and relates to a circRNA marker and a kit for diagnosis and curative effect evaluation of depression. The present invention provides a circRNA marker for the diagnosis of depression, including a combination of four circRNAs hsa_circRNA_002143, hsa_circRNA_103636, hsa_circRNA_100679 and hsa_circRNA_104953, the primer sequences of the above four circRNAs are shown in SEQ ID NO.1 to SEQ ID NO.8 shown; probes for the above four circRNAs are also provided, and the probe sequences are shown in SEQ ID NO.9 to SEQ ID NO.12. The present invention also provides a circRNA marker for evaluating the curative effect of antidepressant drugs. The beneficial effect of the present invention is that for the first time a biomarker for depression and a circRNA biomarker for evaluating the efficacy of antipsychotic drugs are provided. After clinical verification, it has high specificity and sensitivity, and has a high diagnostic reference value. As a specific biomarker for depression, circRNA will bring about revolutionary changes in the diagnosis and treatment of depression.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker, a kit and a gene chip for the diagnosis of depression. Background technique [0002] The core symptoms of depression are low mood, loss of interest, and lack of energy. More than 350 million people worldwide suffer from depression. The World Health Organization predicts that by 2020, depression will occupy the second place in the world's disease burden. Major depressive disorder is a common mental illness with a lifetime prevalence of about 2% to 14%. Major depressive disorder suicides account for 1 / 3-1 / 2 of all suicides, and their chances of comorbid cardiovascular disease and myocardial infarction are significantly higher than normal people, seriously threatening human mental and physical health. It not only seriously affects the quality of life of patients, but also has a significant impact on their social and occupational functions, burdens society with a heavy ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11C40B40/06
CPCC12Q1/6883C12Q2600/118C12Q2600/158
Inventor 张理义
Owner 张理义