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Salt-resistant ssr molecular marker of fluffy white wax

A technology of molecular labeling and velvety wax, applied in the field of molecular biology

Active Publication Date: 2019-03-26
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the search found that there is no report on the salt-tolerant SSR molecular marker of white wax tomentosa

Method used

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  • Salt-resistant ssr molecular marker of fluffy white wax
  • Salt-resistant ssr molecular marker of fluffy white wax
  • Salt-resistant ssr molecular marker of fluffy white wax

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Extraction of genomic DNA from pewter

[0032] (1) Material

[0033] Salt-tolerant fluff white wax H (Fraxinus velutina Torr) and salt-sensitive fluff white wax S. Take the fresh leaves that have just grown, seal them in a fresh-keeping bag and place them in an ice box to bring them back to the Shandong Academy of Forestry Sciences. After freezing in liquid nitrogen, place them in a refrigerator at -70°C for later use.

[0034] (2) Using CTAB method to extract genomic DNA from leaves

[0035] 1) Place the prepared CTAB extraction buffer in a water bath at 65°C for 30 minutes, and place chloroform:isoamyl alcohol (24:1), absolute ethanol, and 70% ethanol in a refrigerator at -20°C for pre-cooling. The mortar needs to be pre-cooled in advance, and then pre-cooled with a small amount of liquid nitrogen before use.

[0036] 2) Take 0.1-0.2g white wax young leaves, add sufficient liquid nitrogen (add it in two parts) to grind to powder, quickly transfer to a 2ml centrifuge tube, ...

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Abstract

The invention discloses an SSR molecular marker for the salt tolerance of Fraxinus velutina. Primers of loci of the SSR molecular marker for the salt tolerance of Fraxinus velutina include the primer S78 and the primer S95. The SSR molecular marker is expected to become a standard primer for identifying the salt tolerance of Fraxinus, and the accurate molecular marker is provided for rapid identification of salt-tolerant germplasm of Fraxinus and salt-tolerant breeding of Fraxinus. By means of the SSR molecular marker, the purpose of identifying salt-tolerant Fraxinus and salt-sensitive Fraxinus from the perspective of molecules is achieved, and a reference and a basis are provided for research such as salt tolerance molecular identification of Fraxinus.

Description

Technical field [0001] The present invention relates to a molecular marker, in particular to a salt-tolerant SSR molecular marker of villi white wax, belonging to the technical field of molecular biology. Background technique [0002] Salt damage is a common abiotic stress for plants in the Yellow River Delta, and it is one of the main environmental factors affecting plant growth and yield. Therefore, the breeding of new salt-tolerant varieties of plants has always been the focus of research at home and abroad. Plant salt tolerance is a complex quantitative trait, involving the coordination of many genes and multiple salt tolerance mechanisms (Hao Derong et al., 2013). It is very difficult to identify salt-tolerant and salt-sensitive materials through morphological methods, especially to screen out salt-tolerant individuals from the hybrid progeny of salt-tolerant and salt-sensitive plants, which usually requires physiological determination and salt-tolerance test, which is time...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 燕丽萍刘翠兰吴德军王因花姚俊修李丽藏真荣任飞
Owner SHANDONG FOREST SCI RES INST