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A clean preparation method of plant polypeptide/protein

A plant polypeptide and protein technology, which is applied in the field of clean preparation of plant polypeptide/protein, can solve the problems of uncontrollable molecular weight ratio, different effects, large average molecular weight, etc., and achieve controllable molecular weight range, low viscosity and high solubility Effect

Active Publication Date: 2020-01-10
SHANDONG BOAOKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the average molecular weight of plant peptide products on the market is large, and the proportion of molecular weight in a specific range is uncontrollable. However, the mechanism and effect of peptides with different molecular weights are different, so the controllability of their functions is poor.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 prepares plant polypeptide with soybean meal as raw material

[0042] Proceed as follows:

[0043] (1) Raw material pretreatment: firstly, the soybean meal is tested to ensure that there is no corruption and mildew. Then, ultrasonic pretreatment is carried out for 360 seconds, then crushed, passed through a 60-mesh sieve, and set aside.

[0044] (2) Slurry mixing: under stirring conditions, slowly add the above-mentioned pulverized and sieved raw materials into the sodium hydroxide solution with a pH value of 9.5, and the solid-to-liquid ratio is 1:10 (mass ratio).

[0045] (3) Extraction and separation: under stirring conditions, raise the temperature to 55±2°C, and react for 2.5 hours to fully extract; after the reaction, separate the solid and liquid to obtain a supernatant for later use.

[0046] (4) Purification: adjust the pH of the above supernatant to 5, let stand to allow the protein to fully flocculate, then separate the solid and liquid to obtai...

Embodiment 2

[0052] Example 2 Preparation of Plant Polypeptides Using Peanut Meal as Raw Material

[0053] Proceed as follows:

[0054] (1) Raw material pretreatment: firstly, the peanut meal is tested to ensure that there is no corruption and mildew. Then, ultrasonic pretreatment is carried out for 600 seconds, then crushed, passed through an 80-mesh sieve, and set aside.

[0055] (2) Slurry mixing: under stirring conditions, slowly add the above-mentioned pulverized and sieved raw materials into the alkali solution sodium hydroxide solution with a pH value of 9.5, and the solid-to-liquid ratio is 1:12 (mass ratio).

[0056] (3) Extraction and separation: under stirring conditions, raise the temperature to 55±2°C, and react for 3 hours to fully extract;

[0057] (4) Purification: adjust the pH of the above supernatant to 4.5, let stand to allow the protein to fully flocculate, then separate the solid from the liquid to obtain the crude protein, wash twice with 95% ethanol solution, remov...

Embodiment 3

[0063] Embodiment 3 prepares plant polypeptide with corn yellow powder as raw material

[0064] Proceed as follows:

[0065] (1) Raw material pretreatment: First, the corn yellow powder is tested to ensure that there is no corruption and mildew. Then, ultrasonic pretreatment is carried out for 380 seconds, then pulverized, passed through a 100-mesh sieve, and set aside.

[0066] (2) Slurry mixing: under stirring conditions, slowly add the above-mentioned pulverized and sieved raw materials into the sodium hydroxide solution with a pH value of 9.5, and the solid-to-liquid ratio is 1:8 (mass ratio).

[0067] (3) Extraction and separation: under stirring conditions, raise the temperature to 55±2°C, and react for 3 hours to fully extract;

[0068] (4) Purification: adjust the pH of the above supernatant to 4.5, let stand to allow the protein to fully flocculate, then separate the solid from the liquid to obtain the crude protein, wash twice with 95% ethanol solution, remove alcoh...

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Abstract

The invention discloses a clean preparation method for plant polypeptide / protein with a controllable molecular weight interval. The method comprises the following steps of 1, pretreatment of raw materials; 2, pulp mixing; 3, extraction and separation; 4, purification to obtain protein liquid; 5, enzymolysis, wherein the protein liquid is heated for denaturation, Na2SO3 is added, then protease is added, and protease is selected from Alcalase, neutral protease AS1398, pepsin and papain; 6, debittering of polypeptide; 7, separation through a nanofiltration membrane to obtain a polypeptide solution; 8, drying and dewatering to obtain the polypeptide product. The product prepared through the method has the controllable molecular weight interval and the advantages of high solubility, low viscosity, acid soluble stability, low bitterness and the like. The product can be widely applied to food, sport food, curative healthcare food, protein drinks, diet food and novel fermented food.

Description

technical field [0001] The present invention relates to a clean preparation method of plant polypeptide / protein, in particular to a preparation method of preparing vegetable protein by pretreatment, separation and purification using corn, wheat, soybean, etc. The clean preparation method of the prepared polypeptide product with controllable molecular weight range. Background technique [0002] Peptide is formed by the dehydration condensation of two or more amino acids. It is a fragment of protein structure and function, and makes protein have various physiological functions, and it also has strong special physiological functions. It is formed by dehydration condensation of 2 or 3 amino acids, which become dipeptides and tripeptides respectively, and so on, tetrapeptides, pentapeptides, etc. Generally speaking, if the number of amino acids on the peptide chain is less than 10, it is an oligopeptide, if it reaches 10-50, it is a polypeptide, and if it is more than 50, it is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/34
CPCC07K1/30C07K1/34C12P21/06
Inventor 刘钦松庄新霞刘孟刚张念强许彤陈小刚刘庆忠
Owner SHANDONG BOAOKE BIOTECH
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