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Method for simultaneously obtaining whole brain neural information and co-localization cell construction

A co-localization and cranial nerve technology, applied in the preparation of test samples, instruments, measuring devices, etc., can solve the problems of increasing the complexity of the experiment, the influence of the uniformity of the whole brain signal, and the inability to display the simultaneous acquisition of the whole brain neural structure, etc. , to achieve the effect of avoiding complicated dyeing preparation, avoiding positioning errors, and uniform dyeing effect

Active Publication Date: 2016-09-07
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

However, this protocol requires additional sample handling, and up to 7 days of immersion increases experimental complexity
Surface brain tissue stains more strongly than deep brain tissue, resulting in compromised brain-wide signal homogeneity
In addition, light-transparent samples can currently only use light-sheet illumination micro-imaging systems to obtain data, which is limited by the technical limitations of light-sheet illumination micro-imaging systems. Simultaneous acquisition of neural architecture and cellular architecture

Method used

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  • Method for simultaneously obtaining whole brain neural information and co-localization cell construction
  • Method for simultaneously obtaining whole brain neural information and co-localization cell construction
  • Method for simultaneously obtaining whole brain neural information and co-localization cell construction

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments.

[0027] Such as figure 1 As shown, the present invention simultaneously obtains the whole brain nerve information and co-localized cell construction includes the following specific steps:

[0028] Step S1: Processing the initial sample section.

[0029] Such as figure 2 As shown, a sample 2 containing a labeled nerve structure 1 is fixed in a water tank 3, and the water tank 3 is filled with a processing fluid 4 containing a dye. The sample 2 is completely submerged by the processing fluid 4, and the tool 5 processes the sample 2 until the initial surface to be imaged of the sample 2 is exposed to generate a flat sample section.

[0030] Step S2: Real-time cell structure staining of the sample section.

[0031] Such as image 3 As shown, after a flat sample section is generated, the processing fluid 4 penetrates into the sample from the sample section. Af...

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Abstract

The invention discloses a method for simultaneously obtaining whole brain neural information and co-localization cell construction. The method comprises the following steps: processing a sample by a cutting module to generate a flat fracture surface; painting the fracture surface by a processing liquid so as to make dye molecules in processing liquid penetrate into the deep part of the sample to dye the superficial layer of the sample; subjecting the superficial part to be imaged of the sample to a dual-color imaging treatment by an imaging module; lifting the sample to a certain height, cutting the imaged part to expose a new surface to be imaged; repeating the steps mentioned above until the information of whole sample is obtained; processing the obtained information by a computer, and then establishing a dual-channel three-dimensional dataset layer by layer, wherein one channel represents the neural information, the other channel represents the cell construction information of the sample, and two channel data are precisely matched. According to the method, during the sample processing process, the superficial layer part of a sample labeled with neural information is subjected to real-time cell construction dyeing and dual-color imaging so as to simultaneously obtain whole brain neural information and co-localization cell construction.

Description

Technical field [0001] The invention relates to the acquisition of brain information, in particular to a method for simultaneously acquiring whole brain nerve information and co-localization cell construction. Background technique [0002] The brain is one of the most complex systems in nature, dominating all human activities. Exploring the brain, understanding people themselves, and coping with the challenges of serious brain diseases have always been one of the most important research goals of mankind. There are about 10 in the human brain 11 Neurons of specific molecular type, they pass about 10 14 The connection points (ie synapses) between the neurons are intertwined with each other to form a large number of three-dimensional neural circuits for neural information transmission, processing and integration. The structure of the brain is the basis for the realization of advanced brain functions. The realization of specific brain functions requires the synergy of local and lon...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/30
CPCG01N1/30G01N21/84
Inventor 骆清铭龚辉袁菁李向宁
Owner HUAZHONG UNIV OF SCI & TECH
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