Simultaneous acquisition of whole-brain neural information and method of colocalization cell construction

Abstract

The invention discloses a method for simultaneously obtaining whole brain neural information and co-localization cell construction. The method comprises the following steps: processing a sample by a cutting module to generate a flat fracture surface; painting the fracture surface by a processing liquid so as to make dye molecules in processing liquid penetrate into the deep part of the sample to dye the superficial layer of the sample; subjecting the superficial part to be imaged of the sample to a dual-color imaging treatment by an imaging module; lifting the sample to a certain height, cutting the imaged part to expose a new surface to be imaged; repeating the steps mentioned above until the information of whole sample is obtained; processing the obtained information by a computer, and then establishing a dual-channel three-dimensional dataset layer by layer, wherein one channel represents the neural information, the other channel represents the cell construction information of the sample, and two channel data are precisely matched. According to the method, during the sample processing process, the superficial layer part of a sample labeled with neural information is subjected to real-time cell construction dyeing and dual-color imaging so as to simultaneously obtain whole brain neural information and co-localization cell construction.

Description

technical field [0001] The present invention relates to the acquisition of brain information, and specifically refers to a method for simultaneously acquiring whole brain nerve information and colocalizing cell construction. Background technique [0002] The brain is one of the most complex systems in nature, governing all human activities. Exploring the brain, understanding people themselves, and responding to the challenges of major brain diseases have always been one of the most important research goals of human beings. There are about 10 in the human brain 11 molecular type-specific neurons that pass through approximately 10 14 The connection points (that is, synapses) between neurons are intertwined with each other to form a three-dimensional neural circuit for the transmission, processing and integration of a large number of neural information. The structure of the brain is the basis for the realization of the advanced functions of the brain. The realization of spe...

AI Technical Summary

Problems solved by technology

However, this protocol requires additional sample handling, and up to 7 days of immersion increases experimental complexity

Surface brain tissue stains more strongly than deep brain tissue, resulting in compromised brain-wide signal homogeneity

In addition, light-transparent samples can currently only use light-sheet illumination micro-imaging systems to obtain data, which is limited by the technical limitations of light-sheet illumination micro-imaging systems. Simultaneous acquisition of neural architecture and cellular architecture

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