Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano-material-based detection method for heroin in biological samples

A technology for biological testing materials and testing methods, applied in material testing products, analytical materials, biological testing and other directions, can solve the problems of unstable process and low testing stability, and achieve cheap and convenient, strong surface plasmon resonance performance, biological good compatibility

Active Publication Date: 2016-09-07
CHINESE PEOPLE'S PUBLIC SECURITY UNIVERSITY +1
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gold nanoparticles change color when they aggregate, and can be used for colorimetric analysis, but this process is unstable, and the aggregation state and color change with time, so the detection stability is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano-material-based detection method for heroin in biological samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of nano-gold composite material

[0031] Take 100mL of 0.2% chitosan solution containing 1% acetic acid and place it in a round bottom flask. Heat to 100℃ to keep boiling and stir vigorously. Quickly add 0.2mL 0.1M chloroauric acid aqueous solution to the vigorously boiling solution and stir. After that, stop heating, cool to room temperature naturally, take out 10mL and adjust the pH to 7-9, then mix with 0.5mL 2mg / mL heroin bovine serum albumin at room temperature for 30-60min, wash with physiological saline several times, centrifuge and discard After removing the supernatant, add 2 mL of physiological saline to obtain the nano-gold composite material.

Embodiment 2

[0032] Example 2 Preparation of nano-gold composite material

[0033] Take 200mL of 0.2% chitosan solution containing 1% acetic acid and place it in a round bottom flask. Heat to 100℃ to keep boiling and stir vigorously. Quickly add 0.2mL of 0.1M chloroauric acid aqueous solution to the vigorously boiling solution and stir. After that, stop heating, cool to room temperature naturally, take out 5mL to adjust the pH value to 7-9, and then mix with 0.5mL 2mg / mL heroin bovine serum albumin at room temperature for 30-60min, wash with physiological saline several times, centrifuge and discard After removing the supernatant, add 1 mL of physiological saline to obtain the nano-gold composite material.

Embodiment 3

[0034] Example 3 Preparation of magnetic nanocomposite material

[0035] Take the 0.2mol / L ferric chloride and 0.1mol / L ferrous sulfate mixed solution 100mL and pour nitrogen into it for 30min, add 28% ammonia water to adjust the pH to 10, heat the reaction at 60-90℃ for 20-40min, then centrifuge at 8000rpm 20min, after discarding the supernatant, add 100mL of physiological saline to obtain the magnetic particle solution, then add 100mL of 0.2% polylysine solution, ultrasound at room temperature overnight, centrifuge at 8000rpm for 20min to discard the supernatant, add 100mL of physiological saline resuspension solution . Add 0.5mL 5% glutaraldehyde solution and 1mL 10mg / mL anti-heroin antibody solution to the above solution, shake for 60-120min at 4℃, then add 5mL 5% bovine serum albumin solution for 30min at room temperature, centrifuge at 8000rpm for 20min, After discarding the supernatant, add 10 mL of physiological saline to obtain the magnetic nanocomposite material.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a nano-material-based detection method for heroin in biological samples. The method comprises the following steps: separately preparing a nano-gold composite and a magnetic nanocomposite; mixing the nano-gold composite and the magnetic nanocomposite with a biological sample; and detecting the intensity changes of the ultraviolet-visible absorption spectrum of mixed liquor so as to realize qualitative and quantitative analysis of existence or content of heroin in the biological sample. The nano-material-based detection method is high in reaction efficiency, good in selectivity and environmental adaptability and high in stability and can reach low-and-medium limit detection levels prescribed in the national standards in China.

Description

Technical field [0001] The present invention is a fast test in drugs, and it involves a specifically to detect heroin in biomagitics in nanomaterials. Background technique [0002] The flood of drugs has become a worldwide social problem. It is tied with AIDS and terrorist activities to become the three major public harms on the planet. At the same time, drug crimes are one of the roots of other crimes.According to the data of the global drug report in recent years, the number of global drug drugs has increased from 167 million in 2009 to 324 million in 2012, showing a trend of aging; the 2014 World Drug Report pointed out that the market for drugs is expanding, production is production, productionThe amount, the amount and consumption of consumption are increasing, and the new market is under development.Drug types are opium, cocaine, cannabis, and phenotrophals, and the types of new spiritual activated substances have surged. [0003] Complex operation methods, long -term pre -...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/94G01N21/33
CPCG01N21/33G01N33/9486G01N2021/3155
Inventor 廉洁高云华邱玉琴张锁慧
Owner CHINESE PEOPLE'S PUBLIC SECURITY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com