Recombinant peste des petits ruminants virus N protein and expression method and application thereof

Abstract

The invention provides a recombinant peste des petits ruminants virus N protein and an expression method and an application thereof. The recombinant peste des petits ruminants virus N protein has the amino acid sequence shown in SEQ ID NO:1. The invention also provides a CHO-PPRV-N cell line for stably expressing the recombinant peste des petits ruminants virus N protein, wherein the CHO-PPRV-N cell line has the preservation number of CGMCC No.12281. The invention further provides a kit used for detecting peste des petits ruminants and containing the recombinant peste des petits ruminant virus N protein.

Description

technical field [0001] The present invention belongs to the field of biology. Specifically, the present invention relates to a recombinant Peste des Petits Ruminants virus N protein and its expression method and application. The present invention also provides a CHO-PPRV-N cell line stably expressing the recombinant Peste des Petits Ruminants virus N protein, and its preservation number is CGMCC No.12281. Background technique [0002] Peste des petits ruminants (PPR) is an acute, highly contagious viral infectious disease mainly infecting small ruminants such as goats and sheep caused by Peste des petits ruminants virus (PPRV). High morbidity and mortality, up to 100% and 90%, respectively (Abubakar, et al., 2008). It is also known as sheep plague, and it is an important animal disease prevalent in regions with a large amount of small ruminants such as Africa and Asia (Awa DN, et al., 2000; Roeder PL, et al., 1994; Shaila MS, et al. al., 1996). Because PPR can cause huge...

AI Technical Summary

Problems solved by technology

In 2007, the disease was first introduced into the Ngari region of Tibet, my country, and in recent years (December 2013-May 2014) broke out frequently in various parts of my country, and at least 15 provinces were affected by it (Banyard, et al., 2014) ( figure 1 ), and the spread of the epidemic is fast, the span is large, and the risk is high, which has caused huge economic losses to my country's animal husbandry production and brought a serious threat to the prevention and control of the epidemic

Among them, the immunocapture ELISA diagnostic method developed by the French OIE reference laboratory (CIRAD-EMVT, France) is currently an internationally accepted PPRV antigen detection method (Libeau, et al., 1994), but it has a higher application cost

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