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A bioreactor for static fermentation of Cordyceps militaris and fermentation method thereof

A bioreactor, static fermentation technology, applied in specific-purpose bioreactors/fermenters, bioreactor/fermenter combinations, microorganism-based methods, etc., can solve the problem of not enhancing the mass transfer rate of static fermentation, Limit the culture volume and fermentation scale, the height of the medium is small, etc., to achieve the effect of increasing the fermentation scale, reducing the difficulty, and increasing the fermentation volume

Active Publication Date: 2018-01-09
贵州酵德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the slow mass transfer rate and small medium height in this static fermentation method, the actual culture volume and fermentation scale are greatly limited.
However, there are no bioreactors and corresponding fermentation methods that enhance the static fermentation mass transfer rate at present.

Method used

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  • A bioreactor for static fermentation of Cordyceps militaris and fermentation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: A novel bioreactor for static fermentation of Cordyceps militaris.

[0030] Such as figure 1 Shown, a kind of bioreactor of static fermentation of Cordyceps militaris, it comprises the wide-mouthed glass container 1 that is arranged in the magnetic stirring constant temperature water bath, the bottom of wide-mouthed glass container 1 is provided with inverted stainless steel mesh 2, the inverted stainless steel mesh 2 The top plane of the sieve 2 has a mesh 6, the bottom of the wide-mouth glass container 1 and the inside of the stainless steel sieve 2 are provided with a magnetic stirrer 3, and the stainless steel sieve 2 is provided with a sterile agar gel layer 4. The agar The gel layer 4 will completely cover the top plane of the inverted stainless steel screen 2, and the top of the wide-mouth glass container 1 is wrapped with 6 layers of gauze 5.

[0031] Wherein, the wide-mouth glass container 1 is a 500mL glass beaker, which is equipped with a Φ8mm×...

Embodiment 2

[0033] Embodiment 2: A novel bioreactor for static fermentation of Cordyceps militaris.

[0034] Such as figure 1 Shown, a kind of bioreactor of static fermentation of Cordyceps militaris, it comprises the wide-mouthed glass container 1 that is arranged in the magnetic stirring constant temperature water bath, the bottom of wide-mouthed glass container 1 is provided with inverted stainless steel mesh 2, the inverted stainless steel mesh 2 The top plane of the sieve 2 has a mesh 6, the bottom of the wide-mouth glass container 1 and the inside of the stainless steel sieve 2 are provided with a magnetic stirrer 3, and the stainless steel sieve 2 is provided with a sterile agar gel layer 4. The agar The gel layer 4 will completely cover the top plane of the inverted stainless steel screen 2, and the top of the wide-mouth glass container 1 is wrapped with 6 layers of gauze 5.

[0035] Wherein, the wide-mouth glass container 1 is a 100mL glass beaker, which is equipped with a Φ8mm×...

Embodiment 3

[0037] Embodiment 3: A novel bioreactor for static fermentation of Cordyceps militaris.

[0038] Such as figure 1 Shown, a kind of bioreactor of static fermentation of Cordyceps militaris, it comprises the wide-mouthed glass container 1 that is arranged in the magnetic stirring constant temperature water bath, the bottom of wide-mouthed glass container 1 is provided with inverted stainless steel mesh 2, the inverted stainless steel mesh 2 The top plane of the sieve 2 has a mesh 6, the bottom of the wide-mouth glass container 1 and the inside of the stainless steel sieve 2 are provided with a magnetic stirrer 3, and the stainless steel sieve 2 is provided with a sterile agar gel layer 4. The agar The gel layer 4 will completely cover the top plane of the inverted stainless steel screen 2, and the top of the wide-mouth glass container 1 is wrapped with 6 layers of gauze 5.

[0039]Wherein, the wide-mouth glass container 1 is a 1000mL glass beaker, which is equipped with a Φ9mm×...

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Abstract

The invention discloses a bioreactor for standing and fermenting cordyceps militaris. The bioreactor comprises a wide-mouth glass container which is arranged in a magnetic stirring constant temperature water bath pot; an inverted stainless steel screen mesh is arranged at the bottom of the wide-mouth glass container; magnetic stirrers are arranged at the bottom of the wide-mouth glass container in the stainless steel screen mesh; a sterile agar gel layer is arranged on the stainless steel screen mesh; the top of the wide-mouth glass container is wrapped with gauze. The invention also discloses a method for standing and fermenting the cordyceps militaris by utilizing the bioreactor. According to the method, the limit to the height of a culture medium in existing standing and fermenting of the cordyceps militaris is overcome, and the practical fermenting volume is increased, so that cordycepin can be produced by performing industrial large-scale standing and fermenting on the cordyceps militaris, and the capacity of the cordycepin is greatly improved. Therefore, the method has very high practical application value.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a bioreactor for static fermentation of Cordyceps militaris and a fermentation method thereof. Background technique [0002] Cordycepin is one of the main active ingredients in Cordyceps. Cordycepin has a variety of biological activities, such as anti-tumor, anti-proliferation, anti-metastasis, antibacterial, antiviral, immune regulation, and anti-inflammation. The preparation of cordycepin mainly includes chemical synthesis and biosynthesis. Due to the high production cost of chemically synthesized cordycepin, complex synthesis process, low yield and difficult product purification, cordycepin is mainly prepared by biosynthesis. There are two ways to prepare cordycepin by biosynthesis: one is to obtain Cordyceps fruiting body by solid fermentation, and then extract it; the other is to directly extract from the fermentation broth through liquid ferment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/24C12M1/02C12P19/40C12R1/645
CPCC12M23/08C12M27/02C12M41/18C12P19/40
Inventor 汤佳鹏金柳函孔婷
Owner 贵州酵德生物科技有限公司