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Waldheimia glabra volatile oil and application thereof in pharmacy

A kind of the technology of flavonoid chrysanthemum and volatile oil

Inactive Publication Date: 2016-10-05
TIBET UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the current domestic and foreign research work on the anti-virus and anti-inflammatory properties of Tibetan chrysanthemum chrysanthemum (Waldheimia) is blank, although some studies have reported the secondary metabolites and their anti-inflammatory properties of the chrysanthemum chrysanthemum (Waldheimia) plant in the Nepal Kelmbu region. Research work on the change of free radical phenolic compounds with altitude concentration (Annamaria Giorgi), in which headspace solid phase extraction method was used to analyze the chemical composition of volatile organic compounds, but the distribution of plants collected in this study is different from that of the source plants of the present invention, especially the ecological The environment is completely different; the main chemical components obtained in this study are different from the volatile oil components extracted from the plant to be provided by the present invention; and the pharmacological activity of the volatile oil has not been reported in this study, etc. And its new application in pharmacy, especially the anti-inflammation and anti-influenza virus activity of the volatile oil of Tibetan chrysanthemum chrysanthemum

Method used

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  • Waldheimia glabra volatile oil and application thereof in pharmacy
  • Waldheimia glabra volatile oil and application thereof in pharmacy
  • Waldheimia glabra volatile oil and application thereof in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Extraction and Preparation of Tibetan Panmangosteen Volatile Oil

[0025] Take the dry whole herb of Tibetan flat mans chrysanthemum (W. glabra), pulverize it with a traditional Chinese medicine grinder, pass it through a (40 mesh) sieve, weigh 100 g, and soak it in distilled water overnight. According to the "National Pharmacopoeia of the People's Republic of China 2010 Edition Appendix XD" volatile oil determination method extraction 4h, get 1.5% light yellow essential oil;

[0026] Carry out chemical composition analysis according to " Chinese National Pharmacopoeia 2010 edition appendix XD " volatile oil determination method; Adopt gas chromatography-mass spectrometry detection (GC-MS) analysis, chromatography-mass spectrometry analysis condition is as follows:

[0027] Detection instrument: American Thermo DSQ gas chromatography-mass spectrometer;

[0028]Chromatographic conditions: Hp-5MS capillary column (5% phenyl methyl siloxane, 30m×0.25mm×0.25μm) c...

Embodiment 2

[0033] Example 2 Screening experiment of anti-influenza virus activity of volatile oil of chrysanthemum chrysanthemum

[0034] Detect the volatile oil of Tibetan chrysanthemum chrysanthemum made by MTT method to MDCK cytotoxicity, and CPE method (cytopathic) detects that the volatile oil of Tibetan chrysanthemum chrysanthemum is to influenza A virus A3 (H3N2) (A3 / Jingke) of influenza virus type A / 30 / 95) (purchased from Shanghai Center for Disease Control and Prevention), inhibitory effect, using ribavirin as positive control experiment:

[0035] First, the cytotoxicity test of the drug was carried out. After the MDCK cells were cultured in the cell culture plate to form a monolayer, different concentrations of the drug solution were added to continue the culture. The cytopathic or MTT staining method was used as the observation index, and the drug effect was calculated by the Reed-Muench method. The maximum concentration at which cells are not toxic (TD 0 ) and the median to...

Embodiment 3

[0039] Example 3 Screening experiment of anti-inflammatory activity of volatile oil of Tibetan chrysanthemum chrysanthemum

[0040] LPS and PolyI:C were used as stimulants to activate the TLR4 and TLR3 receptors of the macrophage cell line RAW264.7, triggering the downstream inflammatory response, and evaluating the anti-inflammatory effects of volatile oils induced by LPS and Poly IC by detecting NO in the cell supernatant The strength of the ability:

[0041] RAW264.7 cells were digested and counted according to 4*10 5 / ml seed plate, prepare 1mg / ml stimulant, 1mg / mlLPS stock solution, 100uM DEX stock solution and 0.5mg / ml volatile oil stock concentration (preparation concentration: 8, 40, 200ug / ml final concentration: 4, 20, 100ug / ml); prepare stock solution concentration: 0.5mg / ml, DMSO: 1:800, add the plate and place it in a cell incubator for culture, then take out and collect the supernatant (180ul / well) for determining the secretion of relevant inflammatory mediator ...

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Abstract

The invention belongs to the field of Tibetan medicine production, and relates to waldheimia glabra volatile oil and the application thereof in pharmacy. The volatile oil extracted from waldheimia glabra mainly comprises bisabolol olefinic terpene, jatamansone, spathulenol, caryophyllene and viridiflorol. Activity detection under different concentration gradients is conducted with the pharmacological screening CPE method and the MTT method, and results prove that the waldheimia glabra volatile oil has high inflammation resistance and anti-influenza virus activity. The waldheimia glabra volatile oil can be used for preparing drugs for resisting inflammation and influenza virus or controlling seasonal flu, especially drugs for controlling human avian influenza, as well as drugs for controlling influenza A(H1N1) and seasonal flu. The waldheimia glabra volatile oil can be also used for preparing antipyretic drugs.

Description

technical field [0001] The invention belongs to the field of Tibetan medicine pharmacy, and relates to the volatile oil of Tibetan chrysanthemum chrysanthemum and its application in pharmacy, in particular to the application of the volatile oil from Tibetan chrysanthemum chrysanthemum in the preparation of anti-inflammatory and antiviral medicines. Background technique [0002] Influenza viruses belong to the Orthomyxoviridae family and are RNA viruses. According to research reports, influenza virus nucleoprotein NP and matrix protein M1 are different in antigenicity, and influenza virus can be divided into three types: A (A), B (B) and C (C), and all three types of influenza virus can infect people. Among them, influenza A virus can be divided into several subtypes according to the structure of its surface hemagglutinin protein (HA) and neuraminidase (NA) and its genetic characteristics. So far, 16 subtypes of HA have been found, H1- H16, NA has 9 subtypes N1-N9. Studies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P29/00A61P31/16C11B9/02
Inventor 德吉卢燕陈道峰
Owner TIBET UNIV
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