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A kind of method for reducing the viscosity of cereal culture medium

A culture medium and grain technology, applied in the direction of fungi, etc., can solve the problems of increasing energy consumption, increasing labor intensity, increasing production costs, etc., and achieve the effect of solving viscosity problems, high water absorption rate, and loose particles

Active Publication Date: 2019-09-10
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The sterilization process disclosed in the above patents combines sterilization and hydration into one step. Although the sterilization effect is achieved, it is difficult to grasp the degree of hydration and the problem of medium viscosity cannot be solved.
Sterilizing the medium after subpackaging not only takes up a lot of sterilization space, increases energy consumption, but also increases labor intensity and increases production costs

Method used

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  • A kind of method for reducing the viscosity of cereal culture medium
  • A kind of method for reducing the viscosity of cereal culture medium
  • A kind of method for reducing the viscosity of cereal culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The preparation of embodiment 1 millet culture medium

[0039] Add water to the millet according to the ratio of 1:0.2 mass ratio of material to water, put it in the steaming tank for sterilization at 121°C for 30 minutes, wait for the steaming material to cool down to 85°C, add sterilized water to the steaming pot to keep the mass ratio of material and water at 1:1.6, temperature 85°C, hydration for 30 minutes. After the hydrated millet is cooled to room temperature, pump the seed liquid of Isaria tenuipes (Isaria tenuipes) into the steaming tank through the pipeline and mix evenly. Cultivation room. medium see Figure 4 .

Embodiment 2

[0040] The preparation of embodiment 2 rice culture medium

[0041] Add sterilized water to the rice according to the mass ratio of feed to water 1:0.3, put it in the steaming tank for sterilizing at 121°C for 25 minutes, wait for the steaming material to cool to 95°C, add sterilized water to the steaming tank to ensure the quality of the feed water The ratio was maintained at 1:1.5, the temperature was 95°C, and the hydration was performed for 25 minutes. After the hydrated rice is cooled to room temperature, pump the seed liquid of Ophiocordyceps odonatae into the steaming tank through the pipeline and mix evenly. Under clean conditions, it is divided into the culture tray by the packing machine, and the culture tray is moved into the culture room. nourish. medium see Figure 5 .

Embodiment 3

[0042] The preparation of embodiment 3 corn medium

[0043] Add sterilized water to crushed corn kernels according to the ratio of feed to water mass ratio 1:0.4, put it in a steaming tank for sterilizing at 121°C for 20 minutes, wait for the steaming material to cool to 100°C, add sterilized water to the steaming tank, and make the material The water mass ratio was maintained at 1:1.8, the temperature was 100°C, and the hydration was performed for 25 minutes. After the hydrated corn kernels are cooled to room temperature, pump the seed liquid of Purpureocillium lilacinum into the steaming tank through the pipeline and mix evenly. room culture. medium see Image 6 .

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Abstract

The invention provides a method of reducing viscosity of a cereal culture medium. Sterilization and hydration are separately completed in two steps in a material steaming tank, wherein the mass ratio of cereal to water in the sterilization step is 1:0-1 and the mass ratio of cereal to water in the hydration step is 1:1.5-2. The cereal culture medium is high in water absorption, has loose granules and is free of viscosity. The method has important significance in automatic fertilization on solid fungus and increase on utilization rate of the culture medium.

Description

technical field [0001] The invention relates to a preparation method of a cereal medium, in particular to a preparation method for reducing the viscosity of a cereal medium, and belongs to the field of solid fermentation. Background technique [0002] Fungi are the most commonly used microorganisms for solid-state fermentation, because their hyphae can grow on solid surfaces and penetrate into the substrate, making them more suitable for solid-state fermentation than other single-celled microorganisms. Most fungi adopt solid fermentation to produce high value-added species or produce special structures such as fruiting bodies, conidia or sclerotia. Fermentation raw materials often use grains as culture media to comprehensively utilize fermentation products. However, in the process of large-scale cultivation, the existing sterilization and hydration processes are all combined into one step, and the grain medium is prone to overflow and stick into agglomerates under high-tempe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 李增智谭悠久陈祝安董建飞王玉芹孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD