Synthetic peptide NK3R-A2 based on NK3 receptor and application thereof

Abstract

The invention belongs to the technical field of tumor treatment, and particularly relates to a synthetic peptide NK3R-A2 based on an NK3 receptor. The synthetic peptide NK3R-A2 based on the NK3 receptor has the tumor angiogenesis resisting effect. The molecular weight of the synthetic peptide is 1536.70 Da, and the specific sequence of the synthetic peptide is CNGRCGGDFF(MeF)GLM-NH2. The synthetic peptide is prepared through an Fmoc solid-phase polypeptide synthesis method and can be used for preparing anti-tumor preparations. Through in-vitro cell viability experiments, cell scratch experiments and cell migration experiments, the angiogenesis resisting effect of the synthetic peptide is verified; meanwhile, through further chicken chorioallantoic membrane carrier experiments and euangiotic mice S180 transplantation tumor model experiments on the synthetic peptide, it is verified that the synthetic peptide is obvious in tumor inhibiting effect, has the tumor angiogenesis resisting effect and has no obvious toxic or side effect; the synthetic peptide has good medical application prospects and also provides a targeting sequence with great potential for tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of tumor treatment, and in particular relates to a synthetic peptide NK3R-A2 based on NK3 receptor with anti-tumor angiogenesis effect. Background technique [0002] In recent years, with the rapid development of the global economy, the level of clinical diagnosis, surgical treatment, chemotherapy and radiotherapy has also been continuously improved. In recent decades, the number of cancer patients has increased linearly, and people are in urgent need of new and effective tumor treatment strategies and means. Since Folkman discovered tumor angiogenesis factor (TAF) in the 1970s, people have gained a new understanding of angiogenesis, especially in the past 10 years, people have conducted a lot of research on the relationship between angiogenesis and tumor development, Tumors are found to be vascular-dependent malignant cells, and their growth, metastasis, recurrence and prevention are closely related to tum...

AI Technical Summary

Problems solved by technology

[0004] At present, although there have been many basic studies on the biological regulation mechanism of NKB and its receptor NK3, it is still in the preliminary exploration stage, and further research on its application in anti-tumor angiogenesis still needs to be further explored.

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